L-精氨酸与N-硝基L-精氨酸甲基酯对雄性大鼠睾酮分泌的影响

The Study Effect of the N-nitro-L-arginine-mythel-ester and L-arginine on the in Vivo Release of Testosterone in Male Rats.

目的:阐明一氧化氮(NO)对男性生殖轴中睾丸内分泌功能的调节作用。材料与方法:于雄性SD大鼠腹腔内注射不同剂量(0～210mg/kg)的L-ARG(L-arginine,L-ARG,NO前体)与L-NAME(Nw-nitrio-L-arginine-mythel-ester,L-NAME,NO合酶的竞争性抑制剂,0～70mg/kg),每天一次,共8天,最后一次注射后1小时采血 然后将SD大鼠分成4组,分别注射同一剂量的L-ARG(160mg/k8)、L-NAME(50mg/kg)、L-ARG+L-NAME和生理盐水,每天一次,注射13天,每隔3～4天采血一次。用放射免疫同步测定法测定血清中睾酮含量,对所得数据进行统计学处理。结果:L-ARG对睾酮分泌具有抑制作用(P<0.01),而L-NAME则促进睾酮的分泌(P<0.01),两者都具有时间依赖性,并与剂量高度相关。L-ARG和L-NAME混合注射时,睾酮的含量与生理盐水注射组无明显区别(P>0.05)。结论:NO对雄性大鼠的睾酮分泌具有抑制作用。

Objective: To determine if the endocrine of testis in male reproductive axle was regulated by nitric oxide. Materials and Methods : Male rats were injected intraperitoneally L-arginine(L-ARG, 0 - 210mg/kg)and N-nitro-L-arginine-mythel-ester(L-NAME,0~70mg/kg)for 8 days (once a day). The blood samples were collected at one hour after injection. Then four groups of ten male rats were injected intraperitoneally one of following agents (once a day for 13 days): (1)L-ARG (160mg/kg); (2)L-NAME(50mg/kg);(3)L-ARG + L-NAME;(4)Normal saline. The blood samples were collected from tail every 3-4days at 1 hour postinjection. The concentration of serum testosterone was measured with radioimmunoassay. The data statistics were processed with SPSS software. Results : L-ARG suppressed testosterone secretion,while L-NAME stimulated testosterone secretion(P<0. 01). Both have time-dependent and dosedependent. Compared with control group ,L-ARG injected simultaneously with L-NAME did not change concentration of testosterone. Conclusion: No suppressed testoserone secretion.