中国鲎胚胎发育研究

Studies on the Embryo Development of Horseshoe Crab, Tachypleus tridentatus

利用人工授精技术孵化中国鲎 ,详细观察研究中国鲎胚胎早期发育过程的形态变化特点及其规律 .中国鲎的胚胎早期发育可分为卵裂和囊胚形成期 (所需时间约 6d)、原肠胚和附肢原基形成期 (约 11d)、组织分化期 (约 17d)、快速生长期 (约 16d) .在组织分化期 ,胚体共进行 3次胚内蜕皮 ,并在第 2次蜕皮的同时卵胶膜破裂蜕去 ,完成 3次胚内蜕皮的胚体身体扁平 ,各组织器官基本成型定位 .在快速生长期内 ,胚体完成最后一次胚内蜕皮 ,蜕皮后胚体进一步扁平 ,各组织器官进一步完善 .第 4次蜕皮后 35d时间胚体破坏内卵膜而孵化为自由游泳的一龄幼鲎 ,开始胚后发育 .孵化的一龄幼鲎大约经过

By using artificial insemination, the embryo development of horseshoe crab, Tachypleus *@tridentatus was investigated.The embryo development of T.tridentatus can be divided into following stages:period of cleavage and formation of blastula(it takes about 6 d from fertilization), period of gastrula and appendix rudiment(about 11 d), period of tissue differentiation(about 17 d)and period of fast growth period (about 16 d). During the period of tissue differentiation, embryo exuviates third times, rupturing of chorion occurs almost as the second molting for embryo happens. Embryo becomes flat and orientation for tissues and organs are determined as third molting of embryo finished. Embryo finishes the four molt is last molt for embryo, it happens in period of fast growth. The embryo hatch out after 3～5 d of last molting of embryo and becomes motile first-instar larve. It is about 180 d that first-intar larvae becomes second-instar juvenile without food.