不同培养基对牛体外受精胚发育率及抗冻性的影响

EFFECT OF DIFFERENT CULTURE MEDIA ON THE DEVELOPMENT RATES AND FREEZING VIABILITIES OF BOVINE IN VITRO FERTILIZED EMBRYOS

采用M199、SOFaa(aa:Amino Acid)和SOF对牛体外受精卵进行发育培养,比较了三种培养条件下的囊胚发育率、孵化率以及这三种条件下培养出来的囊胚的抗冻能力。在M199和SOFaa处理组,囊胚发育率分别为22.6％和29.0％,孵化率分别为 11.3％和8.1％,两者间无显著差异,但均极显著高于SOF处理组(13.6％,0,P<0.01)。三个处理组的囊胚经冷冻解冻后的存活率分别为78.5％、46.4％和17.3％,孵化率分别为41.9％、23.2％和0,三者间存在极显著差异(P < 0.01)。结果表明,不同的培养液不但影响胚胎发育率,同时也影响胚胎的抗冻能力,M199的培养效果优于SOF,氨基酸能明显提高胚胎发育率及抗冻能力。

Bovine IVF embryos were cultured in M199,SOF+ Amino Acids(SOFaa)and SOF respectively, their development rates and the freezing viabilities of blastocysts derived from the three culture media were compared. The blastocyst rates in M199 and SOFaa were 22.6% and 29.0%, the hatching rates of blastocysts were 11.3% and 8. 1% , respectively. They were significantly higher than that (13.6% and 0)in SOF group(p<0.01).The survival rates of blastocysts derived from Ml99,SOFaa and SOF after freezing-thawing were 78.5% ,46.4% and 17. 3% ,the hatching rates were 41.9% ,23.2% and 0. There were significant differences among them(P< 0.01).The results indicated that the different culture media affected not only the development rates but also the freezing viabilities of blastocysts. The culture efficiency of IVF embryos in M199 was better than that in SOF. Amino Acids can enhance the development rates and the freezing viabilities of blastocysts.