保持培养细胞原位、原形的超薄切片制备法

AN UITRATHIN SECTIONING METHOD OF MAINTAINING THE LOCATION AND SHAPE OF CULTURED CELLS

本文介绍先用环氧树脂Epon812包埋剂制成丘状膜,再在膜上培养细胞,直接将膜与细胞一起包埋,制备超薄切片。此种方法不仅能克服以往培养细胞需要离心成团,容易造成细胞破碎、变形的缺点;还能避免琼脂预包埋法悬浮细胞造成的抗原封闭;并能得到为数较多的连续超薄切片。它操作简便,较好地保持了培养细胞生长的原来位置及原有形状,为培养细胞进行免疫电镜的操作和提高制备培养细胞超薄切片的数量和质量提供了一种有效的途径。

In this paper a new kind of method for preparing ultrathin sections was introduced. First,Epon812 was used to make colliculus membrane in a plate, and then cells were cultured on it. When the density of the cells was fit for preparing ultrathin sections, the cells were embedded together with the membrane to make ultrathin sections. Without centrifugation, this method can keep the cells' original shape and location. Moreover it can avoid the disruption and deformation of the cells and the blocking of cellular membrane antigens. Thus it shows an advantageous method for electron microscopic immunocytochemistry and a valid guarantee for the quality and quantity of the ultrathin sections of the cultured cells.