MDR1基因在睫状体色素上皮细胞容积激活性氯电流中的作用(英文)

The role of MDR1 gene in volume-activated chloride currents in pigmented ciliary epithelial cells

用膜片钳、反义寡核苷酸、免疫荧光及激光共聚焦显微镜等技术 ,研究MDR1基因在牛睫状体色素上皮(pigmentedciliaryepithelial,PCE)细胞容积激活性氯电流中的作用。PCE细胞表达MDR1基因产物 P糖蛋白 (P gp)。反义MDR1寡核苷酸抑制MDR1基因的表达 (P gp免疫荧光减少 93 % ) ,延缓容积激活性氯电流的出现 (潜伏期延长10 9% ) ,并导致激活率降低 62 %及电流峰值减小 5 6%。而核酸转染剂阳离子脂质体和非配对性的寡核苷酸对电流没有显著性影响。上述观察结果表明 。

The role of multidrug resistance (MDR1) gene in the activation of volume activated chloride currents in bovine pigmented ciliary epithelial (PCE) cells was investigated by the patch clamp technique, the antisense approach, the immunofluorescent technique and the confocal microscopy PCE cells express P glycoprotein (P gp, the product of MDR1 gene). An MDR1 antisense oligonucleotide suppressed MDR1 expression (93% reduction of P gp immunofluorescence), delayed the activation of a volume activated chloride current (latency prolonged by 109%), reduced the activation rate by 62% and decreased the peak value of the current by 56% The transfection reagent lipofectin and the mismatch control oligonucleotide did not significantly affect the current The data indicate that the volume activated chloride current is associated with the endogenous expression of MDR1 gene in PCE cells