摘要
采用亲和层析及电泳制备等方法 ,从防已科植物波叶青牛胆 (Tinosporacrispa)根茎中分离到一种胰蛋白酶抑制剂TCTI。对其性质研究表明 :TCTI的相对分子量约为 10 0kD ,对牛胰蛋白酶的抑制常数Ki为 2 0 8× 10 7mol/L (BAPNA为底物时 ) ,摩尔抑制比为 1∶3 5 ,是一种抑制活力很强的竞争性抑制剂。TCTI具有很高的耐热性 ,在 10 0℃加热 60min ,仍保持 93%的抑制活力。另外 。
A trypsin inhibitor named TCTI was isolated from the rhizomes of Tinospora crispa (Menispermaceae)by trichloroacetic acid precipitation,salting out,chromatography on trypsin Sepharose 4B and SHyper TM 10 FPLC column,preparative PAGE and electroelution The relative molecular weight of TCTI was 10 0?kD by TSDS-PAGE The inhibition of trypsin by the TCTI was competitive,with a Ki value of 2 08×10 7 ?mol/L (BANPA as substrate) and the mole ratio was about 1∶3 5 TCTI was heat-tolerating When heated up to 100℃ for 60?min,it still had 93% residual activities On the other hand,TCTI can be easily crystalized under lower temperature
出处
《云南植物研究》
CSCD
北大核心
2002年第1期103-108,共6页
Acta Botanica Yunnanica
基金
云南省自然科学基金资助项目 ( 1999C0 0 77M)
