阴沟肠杆菌B8发酵液对植物的促生作用和IAA分析

Promoting plant growth and IAA analysis of Enterobacter cloacae B8 fermentation liquid

以甜玉米 Sh2和番茄为试验材料 ,研究阴沟肠杆菌 B8发酵液对植物的促生作用 ,并对其所含的主要生理活性物质进行了分析 .结果表明 :以适当浓度的发酵液喷施植株幼苗期的叶面 ,两周后 ,发现其株高与对照相比明显增加 ,根系也较对照发达 . HPL C证实该发酵液中含有植物生长激素吲哚乙酸( IAA) .在最适条件下 ,培养基中添加 0 .2 %色氨酸时 ,发酵液中 IAA含量可达 90 4 .38mg/ L.试验还发现 ,喷施发酵液后的植株内源

WT5”BZ]In this experiment,sweet corn Sh2 and tomato were used to study promoting plant growth of Enterobacter cloacae B8 fermentation liquid, and its main biological active substance was also analyzed. The results showed that the growth of the testing plants could be accelerated after spraying with the suitable concentration of the fermentation liquid. Furthermore, the HPLC method confirmed that the main plant hormone in the fermentation liquid was indole 3 acetic acid (IAA). Under optimum conditions, its content reached 904.38 mg/L in liquid medium when adding 0.2% tryptophan. It was also found the level of endogenous IAA in testing plants was increased. [WT5”HZ]