多区域性高血压与血管紧张素转化酶基因的插入/缺失多态性的关系

Relationships between the hypertension in the multiple areas and the insertion/deletion polymorphism of angiotensin convertion enzyme

目的 :观察肾素血管紧张素系统 (RAS)的关键酶——血管紧张素转化酶 (ACE)基因水平在高血压发病机制中的作用 ,探讨 ACE基因多态性与不同区域高血压之间是否存在相互关系。方法 :选择西藏高原区域(2 9例 )、武汉地区 (2 3例 )、福州沿海地区高血压患者 (36例 )及正常对照组 (各 2 0例 )。对影响 ACE基因表达水平的第 16内含子存在的插入 (I)和缺失 (D)的多态性进行分析。提取患者血清 DNA,采用聚合酶链反应(PCR)技术扩增 ACE基因第 16内含子 ,反应产物在质量分数为 1.5 %的琼脂凝胶上电泳分析 ,检测各地区组血清 ACE基因多态性。对 ACE等位基因频率和各基因型频率进行统计学分析。结果 :高血压组 DD、ID和 II基因型的频率分别为 :福州 :0 .36、0 .5 6和 0 .0 8;武汉 :0 .6 5、0 .30和 0 .0 4;西藏 :0 .34、0 .5 9和 0 .0 7。高血压组等位基因 D和 I的频率分别为 :福州 :0 .6 4和 0 .36 ;武汉 :0 .80和 0 .2 0 ;西藏 :0 .6 4和 0 .36。正常对照组 DD、ID和II基因型的频率分别为 :福州 :0 .2 0、0 .5 0和 0 .30 ;武汉 :0 .2 5、0 .40和 0 .35 ;西藏 :0 .30、0 .45和 0 .2 5。正常对照组等位基因 D和 I的频率分别为福州 :0 .45和 0 .5 5 ;武汉 :0 .45和 0 .5 5 ;西藏 :0 .5 3和 0 .47。结果 ,除武汉地区高?

Objective:To investigate the role of angiotensin convertion enzyme(ACE) gene in the pathogenesis of hypertension and the relationships between ACE gene polymorphism and hypertension in multiple areas.Methods:ACE gene polymorphism of the insertion(I) and deletion(D) on 16th intron in 81 patients with hypertension and 60 healthy people in the areas of Tibet (29 cases),Wuhan (23 cases),Fuzhou (29 cases) was investigated using polymerase chain reaction(PCR) method.The serum DNA was extracted and PCR technique was performed to obtain the 16th intron on ACE gene.Electrophoresis of PCR products was done on 1 5 % agrose gel and the DNA polymorphism of ACE gene was investigated in patients coming from different areas.The frequency of ACE alleles and different genotypes were analyzed statistically.Results:The frequency of occurrence of ACE genotype in patients with hypertension was respectively as follows:DD 0 36,ID 0 56,II 0 08 in Fuzhou;DD 0 65,ID 0 30,II 0 04 in Wuhan;DD 0 34,ID 0 59,II 0 07 in Tibet.The frequency of D and I alleles in patients with hypertension was 0 64 and 0 36 in Fuzhou,0 80 and 0 20 in Wuhan,0 64 and 0 36 in Tibet.The results showed that apart from Wuhan area,where the frequency of DD genotype in hypertension patients was higher than that in control group,there were no magnificent differences of DD genotype between the two groups in the other areas.However,the frequency of D alleles was higher in the hypersensitive group than in control and in normal population.Conclusions:The results suggest that the increase of D alleles frequency may be related with the pathogenesis of hypertension.