水溶液中制备分子印迹聚合物微球及其分子识别特性研究

Study on Recognition Mechanism of Molecularly Imprinted Microsphere Synthesized from Aqueous Solution

采用聚乙烯醇 40 0作为分散剂 ,利用模板分子与功能单体 /聚合物残基之间的离子键 (静电 )相互作用形成复合物 ,用水溶液微悬浮聚合法制备了 4 氨基吡啶 (4 AP)和甲氧苄氨嘧啶 (TMP)分子印迹聚合物微球 .并通过色谱行为表征 ,比较了它们对各自的模板分子作用的强弱 .结果表明 ,采用甲基丙烯酸为功能单体制备的分子印迹聚合物微球 (MIMs) ,对带有氨基的模板分子主要靠离子键 (静电 )相互作用 ,且作用力的大小与氨基的个数有关 .色谱研究表明 ,模板分子中氨基数目越多 ,这种作用越强 .而且这种作用不是简单的加合 ,而是协同增强作用 .

Molecularly imprinted polymer microspheres against 4-aminopyridine (4-AP) and trimethoprim (TMP) were synthesized by aqueous micro-suspension polymerization with polyvinyl alcohol 400 as dispersive reagent. The ionic (electrostatic) interaction between molecule and monomer/polymer was used during synthesizing and recognizing process. The interaction mechanisms were compared between TMP and 4-AP imprinted column. The results showed that the interaction between acidic monomer/polymer and template with amino group mainly depended on the ionic (electrostatic) interaction, of which the strength level relied on the number of amino group in template molecules. The results showed that the interaction was the coordinated interaction, but not the simply added interaction.