豆荚软珊瑚Lobophytum sp.的次生代谢产物研究

Studies on the Secondary Metabolite of the Soft Coral Lobophytum sp.

从海南岛三亚海域采集的软珊瑚Lobophytumsp .中分离得五个甾醇苷 (1)～ (5 ) .通过波谱分析 ,确定它们的化学结构依次为 3′ O 乙酰基 4 O [β D 吡喃木糖苷 ]-孕甾 2 0 烯 3β ,4α 二醇 (1) ,4 O [β D 吡喃木糖苷 ] 孕甾 2 0 烯 3β ,4α 二醇 (2 ) ,4′ O 乙酰基 4 O [β D 吡喃木糖苷 ] 孕甾 2 0 烯 3β ,4α 二醇 (3) ,4′ O 乙酰基 4 O [β D 吡喃阿拉伯糖苷 ] 孕甾 2 0 烯 3β,4α 二醇 (4 )和 4 O [β D 吡喃阿拉伯糖苷 ] 孕甾 2 0 烯 3β,4α 二醇 (5 ) ,其中 1为新化合物 .体外细胞毒性实验表明 :化合物 (1) ,(2 )和 (5 )对SKMG 4 ,Hep G2和CNE2三种人体癌细胞具有抑制作用 .

Five steroidal glycosides (1) similar to (5) have been isolated from die soft coral Lobophytum sp., collected from Sanya Bay, Hainan Island. Base on the spectroscopic methods, their structures were determined to be 3'-O-acetyl-4-O- [beta-D-xylo-pyranosyl-oxy]-pregn-20-en-3beta, 4alpha-diol (1), 4-O-[beta-D-xylo-pyranosyl-oxy]-pregn-20-en-3beta,4alpha-diol (2), 4'-O-acetyl-4-O-[beta-D-xylo-pyranosyl-oxy]-pregn-20-en-3beta,4alpha-diol (3), 4'-O-acetyl-4-O-[beta-D-arabino-pyranosyl-oxy]-pregn-20-en-3beta, 4alpha-diol ( 4), and 4-O-[beta-D-arabino-pyranosyl-oxy]-pregn-20-en-3beta,4alpha-diol (5). Among them, 1 is a new compound. Bioassay showed that 1, 2 and 5 displayed cytotoxicity against human SKMG-4, Hep-G2 and CNE2 cell lines in vitro.