期刊文献+

9.1C3对CD2和CD3诱导的杀伤作用的影响及其机制探讨 被引量:1

Study on the effects and its mechanism of 9.1C3 on CD2 or CD3 mediated cytotoxicities
下载PDF
导出
摘要 目的  探讨9.1C3分子对CD2和CD3诱导的PBMC杀伤作用的影响及其作用机制。方法以活化PBMC为效应细胞,采用重导向杀伤实验(redirected cytotoxicity assay,RCA),观察9.1C3对CD2、CD3介导效应细胞杀伤P815细胞作用的影响。以Jurkat细胞为模型,用相应的抗体刺激细胞,通过荧光分光光度计,测定9.1C3对CD2、CD3诱导[Ca2+]i升高的影响。结果9.1C3 mAb能显著抑制CD2 mAb介导活化PBMC对P815细胞的杀伤作用,但对CD3 mAb介导杀伤的抑制作用较弱。以Jurkat细胞为模型,发现CD2 mAb经羊抗鼠Ig(goat anti mouse Ig, GAM Ig)交联后能诱导[Ca2+]i的升高,当同时加入9.1C3 mAb时,[Ca2+]i的升高受到抑制;CD3 mAb在没有GAM Ig交联时即能引起[Ca2+]i的升高,而9.1C3 mAb对CD3 mAb诱导[Ca2+]i的升高有赖于GAM Ig的交联。结论9.1C3分子对CD2和CD3介导的杀伤的抑制程度不同。抑制杀伤细胞脱颗粒依赖的[Ca2+]i升高可能是9.1C3分子抑制活? Aim To explore the effects and its mechanism of 9.1C3 on CD2 or CD3 mediated cytotoxicities of activated PBMC. Methods The effects of 9.1C3 on CD2 or CD3 induced cytotoxicities were investigated by using redirected cytotoxicity assay, in which activated PBMC were used as effectors, and Fc receptor expressing P815 were used as target cells. The effects of 9.1C3 on CD2 or CD3 mediated [Ca2+]i increase in Jurkat cells were detected by spectrofluorometer. Results CD2 mediated cytotoxicity was inhibited significantly by 9.1C3 mAb, whereas CD3 mediated cytotoxicity was inhibited by 9.1C3 mAb to less extent. CD2 mediates [Ca2+]i increase in crosslinking dependent manner, and [Ca2+]i increase was inhibited in the presence of 9.1C3 mAb. However, CD3 mediates [Ca2+]i increase in crosslinking independent manner, and the increase was only inhibited in the presence of 9.1C3 mAb and crosslinker. Conclusion 9.1C3 can inhibit CD2 or CD3 molecule mediated cytotoxicities to different extent. The inhibition of CD2 mediated [Ca2+]i increase may be one mechanism of inhibitory effect of 9.1C3 on CD2 molecule mediated cytotoxicity. The lower inhibitory effect of 9.1C3 on CD3 molecule mediated cytotoxicity may be due to the absence of crosslinker in the experiment of incubating effect cells with antibodies in RCA.
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2002年第2期169-171,共3页 Chinese Journal of Cellular and Molecular Immunology
基金 国家自然科学基金资助项目 No. 39770381
关键词 活化PBMC 9.1C3 抑制型杀伤细胞受体 单克隆抗体 CD2 CD3 activated PBMC 9.1C3 killing cell inhibitory receptor [Ca2+]i
  • 相关文献

参考文献7

  • 1Gordon FB, Tony T, Perry FB, et al. Human natural killer cells, activated lyphocyte killer cells, and monocytes possess similar cytotoxic mechanisms. Proc Natl Acad Sci USA,1983, 80: 7606-7610.
  • 2欧阳为明,金伯泉,张赟,刘雪松,李琦,夏海滨.9.1C3分子对人NK细胞和T细胞细胞毒作用的抑制效应[J].细胞与分子免疫学杂志,2000,16(2):121-123. 被引量:8
  • 3欧阳为明,金伯泉,李琦,李林,刘雪松,韩卫宁,李德敏,夏海滨.9.1C3分子抑制杀伤性细胞的细胞毒作用及其机制[J].中国免疫学杂志,2000,16(5):235-237. 被引量:5
  • 4Aramburu J, Balboa MA, Ramirez A, et al.A novel functional cell surface dimer (Kp43) expressed by natural killer cells and T cell receptor-gamma/delta+T lymphocytes. I. Inhibition of the IL-2-dependent proliferation by anti-Kp43 monoclonal antibody.J Immunol,1990, 144: 3238-3247.
  • 5Hagmann M. A trigger of natural (and other) killers. Science,1999,285: 645-647.
  • 6Leibson PJ. Signal transduction during natural killer cell activation: inside the mind of a killer. Immunity, 1997, 6: 655-661.
  • 7Gabriella P, Valentino T, Alessandra Z, et al. CD94/NKG2-A inhibitory coplex blocks CD16-triggered Syk and extracellular regulated kinase activation, leading to cytotoxic function of human NK cells. J Immunol, 1999, 162: 7181-7188.

二级参考文献5

  • 1[1]Gordon FB, Tony T, Perry FB, et al. Human natural killer cells, activated lymphocyte killer cells and monocytes possess similar cytotoxic mechanisms[J]. Proc Natl Acad Sci USA, 1983; 80: 7606- 7610.
  • 2[2]Aramburu J, Balboa MA, Ramirez A, et al. A novel functional cell surface dimer (Kp43) expressed by natural killer cells and T cell receptor- gamma/delta+ T lymphocytes. I. Inhibition of the IL- 2- dependent proliferation by anti- Kp43 monoclonal antibody[J]. J Immunol, 1990; 144: 3238- 3247.
  • 3[3]Le Drean E, Vely F, Olcese L, et al. Inhibition of antigen- induced T cell response and antibody- induced NK cell cytotoxicity by NKG2A: association of NKG2A with SHP- 1 and SHP- 2 protein- tyrosine phosphatases[J].Eur J Immunol, 1998; 28: 264- 276.
  • 4[4]Lanier LL. NK cell receptors[J]. Annu Rev Immunol, 1998; 16: 359- 393.
  • 5[5]Lanier LL. Natural killer cells: from no receptor to too many[J]. Immunity, 1997; 6: 371- 378.

共引文献10

同被引文献7

  • 1Burns GF, Triglia T, Bartlett PF, et al. Human natural killer cells,activated lymphocyte killer cells, and monocytes possess similar cytotoxicmechanisms[J]. Immunology, 1983, 80(24): 7606-7610.
  • 2Meyaard L, Adema G, Chang C, et al. Lair-1, a novel inhibitoryreceptor expressed on human mononuclear leukocytes[J]. Immunity, 1997,7(2): 283-290.
  • 3Weiming Ouyang, Dongchu Ma, Boquan Jin, et al. 9.1C3 is identical toLair-1, which is expressed on hematopietic progenitors[J]. Biochem Biophy Commun, 2003, 310: 1236-1240.
  • 4Meyaard L, Hurenkamp J, Clevers H, et al. Leukocyte-associated Ig-likereceptor-1 functions as an inhibitory receptor on cytotoxic T cells[J]. JImmunol, 1999, 162(10): 5800-5804.
  • 5Van der Vuurst de Vries AR, Clevers H, Logtenberg T, et al.Leukocyte-associated immunoglobulin-like receptor-1(LAIR-1) isdifferentially expressed during human B cell differentiation and inhibitsB cell receptor-mediated signaling[J]. Eur J Immunol, 1999, 29(10):3160-3167.
  • 6Lebbink RJ, Talitha de Ruiter, Meyaard L, et al. The mouse homologueof the leukocyte-associated Ig-like receptor-1 is an inhibitory receptorthat recruits Src homology region 2-containing protein tyrosinephosphatase (SHP)-2, but not SHP-1[J]. Immunology, 2004, 172: 5535-5543.
  • 7谢鑫,欧阳为明,薛江楠,张新海,刘飞,庄然,金伯泉.LAIR基因的克隆、原核表达及免疫反应性鉴定[J].细胞与分子免疫学杂志,2003,19(2):118-120. 被引量:5

引证文献1

二级引证文献5

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部