ConA对巨噬细胞活性的影响

Effect of ConA on macrophage activity

目的 探讨ＣｏｎＡ对巨噬细胞（Ｍφ）活性的影响。方法以不同剂量的ＣｏｎＡ腹腔注射处理小鼠，４８ ｈ后收集腹腔Ｍφ做体外培养。分别以扫描及透射电镜观察Ｍφ的形态变化。分别以ＭＴＴ比色法和胸腺细胞增殖试验检测Ｍφ分泌ＴＮＦ－α和ＩＬ－１β的水平，并用免疫细胞化学染色法检测这两种蛋白的表达强度。结果ＣｏｎＡ作用后的Ｍφ，表现为体积增大、指状突起增加和胞浆内线粒体等细胞器增加。ＣｏｎＡ活化的腹腔Ｍφ分泌ＴＮＦ－α和ＩＬ－１β的水平增加，其在Ｍφ胞浆内的表达增强。结论ＣｏｎＡ具有激活Ｍφ增强其产生ＴＮＦ－α和ＩＬ－１β的作用。

AimTo explore the effect of ConA on macrophage(Mφ) activity. Methods Mice were treated with various dose of ConA via intraperitoneal injection(ip). Peritoneal Mφs were collected and cultured in vitro. Morphological changes of Mφs were observed by scanning electron microscope and transmission electron microscope. Levels of TNF-αand IL-1β secreted by Mφs in cultural supernatant were detected by MTT colorimetry and thymocyte proliferation test respectively. Expressions of the two cytokins in Mφs were detected by immunocytochemical staining. Results ConA treated Mφs showed volume expansion, increased fingerlike process ases and intracellular organellae. The levels of TNF-α and IL-1β increased in cultural supernatant and cytoplasm of Mφs. Conclusion ConA can activate Mφs and cause them to secrete and express more TNF-α and IL-1β.