Sertoli细胞FasL和TGF-βmRNA表达在感染时的免疫调节作用

Expression and immune regulation of FasL and TGF-β mRNAs in Sertoli cells from infected rat testis

目的 研究大鼠Ｓｅｒｔｏｌｉ细胞在睾丸局部感染时的免疫调节作用。方法以 解脲脲原体（ＵＵ）和致病性大肠杆菌，直接注入大鼠膀胱模拟上行性感染的途径，分别在感染后１、２ 、３ ｗｋ处死大鼠，从大鼠睾丸组织中分离获得高纯度的Ｓｅｒｔｏｌｉ细胞。然后抽提总ＲＮＡ， 用ＲＴ－ＰＣＲ法比较正常组与ＵＵ感染组、致病性大肠杆菌感染组之间ＦａｓＬ ｍＲＮＡ和ＴＧＦ－β ｍＲＮＡ表达的差异性。结果与正常组相比较在ＵＵ感染后，１～３ ｗｋ ＦａｓＬ ｍＲＮＡ 的表达均升高；ＴＧＦ－β ｍＲＮＡ的表达在１、２ ｗｋ时升高， ３ ｗｋ时下降；而致病性大肠杆菌感染后，ＦａｓＬ ｍＲＮＡ 和ＴＧＦ－β ｍＲＮＡ的表达在１－３ ｗｋ时均升高。 结论大鼠Ｓｅｒｔｏｌｉ细胞在抗感染免疫中，可通过ＦａｓＬ ｍＲＮＡ和ＴＧＦ－β ｍＲＮＡ表达的变化，调节睾丸局部的免疫功能及有助于睾丸免疫豁免的维持。

Aim To investigate the immune regulation of Sertoli cells in the local infection of rats＇ testis.Methods Ureaplasma Urealyticum(UU) and pathogenic E. coli were injected into rat＇s bladder, which mimiced an ascending infectious way, and at the same time culture medium was injected into another rat＇s bladder in control group. The rats were put to death at 1, 2 and 3 weeks after injection, respectively, and then Sertoli cells were separated from rat＇s testis. The comparison of levels of FasL and TGF-β mRNA expression among control, UU-infected and E.coli-infected groups was made by RT-PCR. Results As compared with control group, the levels of FasL mRNA expression for UU-infected and E.coli-infected groups increased significantly at one to three weeks after infection. Such was the case with level of TGF-βmRNA expression in E.coli-infected group. But level of TGF-β mRNA expression in UU-infected group was distinct from that of FasL mRNA expression, that is, after infection it increased slightly and obviously during one to two weeks and decreased evidently at three weeks. Conclusion During anti-infective immunity rat＇s Sertoli cells may regulate immune function of the testis by the aid of changes in FasL and TGF-β mRNA expression levels and contribute to maintaining immune previlege of the testis.