风湿性关节炎患者T细胞表达CCR5和CXCR3的三色流式细胞分析　

Three-color flow cytomertric analysis of the expressions of CXCR3 and CCR5 on T cells from patients with rheumatoid arthritis

目的 在单细胞水平上，研究类风湿性关节炎（ＲＡ）患者滑液及外周血中Ｔ淋巴细胞上ＣＣＲ５及ＣＸＣＲ３的表达，并结合临床资料分析其在ＲＡ发病中的可能作用机制及临床应用。方法 分离１５例ＲＡ患者的滑液单个核细胞（ＳＦＭＣ）、外周血单个核细胞（ＰＢＭＣ），及１５正常人ＰＢＭＣ（对照），以三色荧光素标记物进行流式细胞术分析Ｔ细胞上ＣＣＲ５及ＣＸＣＲ３的表达。结果 与ＰＢＭＣ相比较，ＲＡ患者ＳＦＭＣ中Ｔ细胞上ＣＣＲ５及ＣＸＣＲ３的表达显著增高（特别是ＣＣＲ５）；而ＣＸＣＲ３的表达个体差异较大；与正常人相比较，ＲＡ患者初发或活动期未治时，ＰＢＭＣ中ＣＣＲ５及ＣＸＣＲ３的表达明显增多。 ＣＣＲ５及ＣＸＣＲ３的表达率与患者的ＥＳＲ及ＣＲＰ相关。结论 ＣＣＲ５＋Ｔ细胞积聚在ＲＡ患者的病变关节内，且与ＲＡ的病情密切相关。

Aim To study the expression of chemokine receptors CXCR3 and CCR5 on T cells from patients with rheumatoid arthritis (RA).Methods The expressions of CXCR3 and CCR5 on T cells in synovial fluid(SF) and peripheral blood(PB) mononuclear cells from 15 RA patients and healthy individuals were studied by three-color flow cytometry.Results In the majority of samples ,the percentage of CCR5+and CXCR3+T cells,especially the former,were much higher than that in PB. Additionally ,the percentage of CCR5+or CXCR3+T cells was correlated with the patient＇s ESR and CRP. Conclusion A large number of CCR5+ T cells accumulated in the RA patient′s affected articular cavity and this phenomenon have a close relation with the state of illness.