肿瘤坏死因子受体(TNFR)基因转移增强TNF对肿瘤细胞的杀伤作用

Gene transfer of TNF receptor enhances the tumorcidal activity of TNF

目的：探讨ＴＮＦＲ基因转移对肿瘤细胞表面ＴＮＦＲ表达量的影响，观察其对ＴＮＦ杀伤膀胱肿瘤细胞作用的影响。方法：建立ＴＮＦＲ的逆转录病毒表达载体，通过转染包装细胞ＰＡ３１７产生完整病毒颗粒；膀胱癌ＢＩＵ８７细胞经病毒感染后，检测细胞表面ＴＮＦＲ数量及ＴＮＦ对其杀伤作用的变化。结果：ＴＮＦＲ基因转移前膀胱癌ＢＩＵ８７细胞表面与ＴＮＦ结合的位点数量平均为９１２个／细胞，经病毒感染后，细胞表面与ＴＮＦ结合的位点数量平均为２８７２个／细胞（Ｐ＜０．０５）；ＴＮＦ对经病毒感染的ＢＩＵ８７细胞的杀伤作用较未经病毒感染者有明显增强（Ｐ＜０．０５）。结论：以逆转录病毒为载体的基因转移方法可以提高肿瘤细胞表面ＴＮＦＲ的表达量，从而增强ＴＮＦ对肿瘤细胞的杀伤作用。

Objective:To explore the influence of gene transf er of TNF receptor (TNFR) on tumorcidal activity of TNF.Methods: By constructing retroviral expression vector of TNFR and transfecting package cell PA317 to pro duce a virus that can transfer TNFR;Then use the virus to infect BIU 87 cell li ne .The TNFR number on tumor cell membrane and the tumorcidal activity of TNF in vi tro were detected.Results:The number of TNFR on a BIU 87 cell membr ane was 912/cell before the infection,and increased to 2 872/cel l(P<0.05).The tumorcidal activity of TNF in vitro was also enhanced(P<0.05).Conclusion:Gene transfer can increase the TNFR expre ssion on tumor cell membrane and the tumorcidal activity of TNF can also be impr oved.