血管生成检测在恶性淋巴瘤鉴别诊断中的价值

Significance of angiogenesis in the differential diagnosis of malignant lymphoma

目的：探讨血管内皮生长因子（ＶＥＧＦ）表达和微血管密度（ＭＶＤ）在恶性淋巴瘤（ＭＬ）鉴别诊断中的价值。方法：应用免疫组化ＳＡＢＣ法，观察２９例恶性淋巴瘤（ＭＬ）、２０例转移癌淋巴结和１７例慢性淋巴结炎中ＶＥＧＦ表达和ＭＶＤ计数。结果：ＭＬ组和转移癌淋巴结组的ＶＥＧＦ阳性表达率分别为７２．４％和７５％，显著高于慢性淋巴结炎组（Ｐ＜０．００５），ＭＬ和转移癌淋巴结组ＭＶＤ分别为４０．６１±１４．７２、３７．９５±１７．０４，显著高于慢性淋巴结炎组２８．１３±１６．０２（Ｐ＜０．０５）。结论：ＶＥＧＦ表达和ＭＶＤ在良恶性淋巴结病变中均有差异性，可作为鉴别良恶性淋巴结病变的有价值的指标。

Objective:To investigate the significance of vasc ular endothelial growth factor(VEGF) expression and microvessel density (MVD) in the differential diagnosis of malignant lymphoma(ML).Methods: Immunohistochemical assay (streptoavidin biotin peroxidase complex,SABC) was e mployed to detect the expression of VEGF and MVD in 29 cases of ML,20 cases of c arcinoma metastated lymph nodes,17 cases of chroic lymphoditis,4 cases of norma l lymph nodes.Results:Positive ratio in ML、carcinoma metastated lym p h nodes and chronic lymphoditis were 72.4%、75% and 41.4% respectively;incidence of VEGF expression was much lower in chronic lymphoditis than those in ML and c arcinoma metastated lymph nodes(P＜0.005).MVD in ML group,carcinom a metastated lymph nodes group and chronic lymphoditis group i s 40.61±14.72,37.95±17.04 and 28.13±16.02 respectively. MVD in chro nic lympho ditis group was also lower than those in the other two groups (P＜0. 05 ).Conclusion:VEGF expression and MVD were different in chronic lymph oditis,ML and carcinoma metastated lymph nodes and can be used as useful i ndicators to distinguish chronic lymphoditis from ML and carcinoma metastated lymph nodes.