胃癌组织中p16和CyclinD_1基因的表达及动态观察

Expression of p16 and CyclinD_1 in the mucosa of gastric cancer and dynamic observation

目的：研究ｐ１６、ＣｙｃｌｉｎＤ１在胃癌组织中的表达，探讨其与胃癌发生、发展的关系。方法：采用ｍＲＮＡ原位杂交技术检测ｐ１６、ＣｙｃｌｉｎＤ１在不同胃粘膜病变中的表达。结果：ｐ１６在正常胃粘膜、萎缩性胃炎、不典型增生及胃癌组织中的阳性表达率渐降低，ＣｙｃｌｉｎＤ１的阳性表达率依次递增；ｐ１６的阳性表达率与肿瘤恶性程度呈负相关，ＣｙｃｌｉｎＤ１与癌分化无明显相关性；有淋巴结转移及死亡的胃癌患者ｐ１６阳性表达率明显降低，而ＣｙｃｌｉｎＤ１则明显升高。在８０例胃癌组织中，ｐ１６与ＣｙｃｌｉｎＤ１有交叉共存现象２４例，占３０％；相关分析显示， ｐ１６、ＣｙｃｌｉｎＤ１的阳性表达率呈显著负相关。结论：ｐ１６、ＣｙｃｌｉｎＤ１与胃癌的形成密切相关；两者可单独或协同促进胃癌的发生、发展。

Objective:To study the expression of p16 and Cycl inD1 in the mucosa of gastric carcinoma (GC) and its relationship with the development and occurrence of gast ric carcinoma.Methods:The mRNA hybridization in situ was used to determine the expression of p16 and CyclinD1 in different d iseases of gastric mucosa.Results:In GC,the positive rate of the expression of p16 was lower than in atrophic gastritis(AG),but the positive rate of expression of Cyclind1 was hi gher in atypical hyperplasia(AH).The positive rate of p16 expression correlated w ith carcinoma grade ,but no correlation was found between CyclinD1 expression and carcinoma differentiation. The positive rate of p16 expression was lower in gast ric carcino ma with lymph node metastasis than in carcinoma without metastasis but the expre ssion of CyclinD1was inverse.In the two year follow up,13 patients with GC d ied, p16 was positive and CyclinD1 was negtive in all specimen.Crosscoexiste nce of p16 and CyclinD1 was observed in 24 of all 80 cases.Conclusion:Both p16 and CyclinD1 are closely correlated with gastric carcinogenesis, and contribute to the occurrence and deterioration of GC.