肿瘤坏死因子相关凋亡诱导配体基因对人大肠癌细胞株HT29作用的研究

Action of apoptosis-induced ligand gene in relation to tumor necrosis factor on human colon cancer cell line HT29

目的 探讨肿瘤坏死因子 (TNF)相关的凋亡诱导配体基因 (TRAIL)用于人大肠癌细胞株HT2 9基因治疗的实验研究。方法 将重组腺病毒载体 (Ad)介导的TRAIL基因作用于人大肠癌细胞株HT2 9,通过相差显微镜、MTT比色法和流式细胞仪 ,研究分析其对HT2 9细胞作用的效果。结果Ad/GT TRAIL能引起HT2 9细胞出现明显的形态学改变 ,对HT2 9细胞的生长抑制率和凋亡诱导率分别为 5 4 .3%和 11.1% ;联合Ad/PGK GV16后 ,生长抑制率和凋亡诱导率均显著提高 (P <0 .0 5 ) ,分别为 82 .7%和 2 4 .6 %。结论 Ad/GT TRAIL能有效诱导HT2 9的凋亡从而抑制HT2 9的生长 ,联合Ad/PGK GV16后将显著提高其疗效。

Objective To evaluate the gene therapeutic efficiency of apoptosis inducing ligand(TRAIL ) related to tumor necrosis factor on human colon cancer cell line HT29. Methods Human colon cancer cell line HT29 was transfected with adenovirus mediated TRAI L gene Ad/GT TRAIL. The morphological changes, cell growth and apoptosi s were measured by phase contrast microscope, MTT method and flow cytometry. Results Obvious morphological changes in HT29 cells was induced by Ad/GT TRAIL and Ad /P GK GV16. The cell suppression percentage and the percentage of apoptotic cells were 54.3% and 11.1%, respectively. When used in combination with Ad/PGK GV16 , HT29 was suppressed to 82.7% and the percentage of apoptotic cells was 24.6% . This result showed significantly enhanced therapeutic efficiency on HT29 and thus inh ibiting of its growth ( P < 0.05). Conclusion Ad/GT TRAIL is able to induce apoptosis of HT29 and inhibit its growth. Ad/GT TRAIL shows significantly enhanced therapeutic efficiency for HT29 when used in combination with Ad/PGK GV16.