N-乙酰化转移酶2基因多态与喉癌遗传易感性的研究

Relationship between poly morphism of N-acety ltransferase 2 and genetic suscept ibility to lary ngeal carcinoma

目的 探讨N 乙酰化转移酶 2 (NAT2 )基因多态与喉癌遗传易感性的关系。方法 应用聚合酶链反应 限制性片段长度多态性分析 (PCR RFLP)方法 ,对 6 2例喉癌患者进行研究 ,测定其NAT2基因型 ,按吸烟指数 (SI)不同 ,分层分析患癌风险。并与 5 6例非肿瘤患者进行对照。结果 喉癌组慢乙酰化型基因型频率为 80 .6 % ,对照组为 6 0 .7% ,两者差异有显著性 (χ2 =5 .70 ,P =0 .0 17)。高吸烟剂量组的NAT2慢乙酰化型个体 ,患喉癌风险明显高于低吸烟剂量组 ,比值比 (OR)分别为 5 .6 4和 1.38,95 %可信限 (95 %CI)分别为 1.77～ 17.92和 0 .4 2～ 4 .5 2。结论 NAT2慢乙酰化型个体患喉癌风险增加 ,在喉癌发生过程中 。

Objective To investigate the relationship between poly morp hism of N acety ltransferase (NAT2) gene and genetic susceptibility to lary ngea l carcinoma. Methods A case control study on 62 lary ngeal carcinoma patients and 56 controls was conducted. NAT2 alleles were differentiated by poly merase chain reaction ba sed restriction fragment length poly morphism (PCR RFLP) methods using originall y created PCR primers and genomic DNA extracted from peripheral white blood cell s. Genetic risK for NAT2 genoty pe was analy zed by smoKing index (SI, cigarettes smoKed per day ×y ears of smoKing). Results The frequency of NAT2 slow genoty pe was 80.6% in patients with lary ngeal carcin oma and 60.7% in the controls, the difference of which was statistically sig nificant (χ 2=5.70, P =0.017).The odds ratios were 2.70(95% CI 1.19 ～6.11). Among the individuals with NAT2 slow genoty pe at high level of cigarette smoKing, there was a significantly higher risK of 5.64 (95% CI 1.77～17.92), while t hose at low level were considered the reference group ( OR 1.38, 95% CI 0 .42～4.52). Conclusion NAT2 slow genoty pe increases the risK of susceptibility to lary ngeal carcinoma. The combined effect of NAT2 slow genoty pe and exposure to smoKing is observed during the development of lary ngeal cancer. [