HLA-DR2与中国人1型糖尿病相关性的探讨

A study on the relationship between HLA-DR2 and type 1 diabetes mellitus in Chinese population

目的 研究HLA -DR2与中国人 1型糖尿病的相关性。方法 应用序列特异引物PCR(PCR -SSP)技术检测华南地区汉族 64例 1型糖尿病 (1型DM )患者 (包括 15岁前起病组 17例 ,15～ 3 0岁起病组 3 0例以及 3 0岁后起病组 17例 )和 72例健康对照者的HLA -DR2等位基因。结果 1型DM组和对照组HLA -DR2等位基因频率分别为3 1 3 %和 3 6 6% ,差异无显著性 (P >0 0 5 )。 15岁前起病组、15～ 3 0岁起病组和 3 0岁后起病组中HLA -DR2频率分别为 17 6%、3 0 0 %和 47 1% ,各组间比较及分别与对照组比较差异均无显著性 ,但 15岁前起病组与 3 0岁后起病组比较P =0 0 5 8。HLA -DR2 1型DM组与非HLA -DR2 1型DM组比较DK及 /或DKA发生率较低 (5 5 %及 84% ,RR =0 65 )。对包括本组的 5份华人资料的联合分析显示HLA -DR2与IDDM呈弱负相关 (P <0 0 2 5 ,RR =0 65 )。结论 HLA -DR2与中国人 1型DM发病的关系仍有待大样本研究的证实 ,尤应增加儿童起病 1型糖尿病的研究。HLA -DR2与 1型糖尿病患者DK及

Objective To study the association of HLA-DR2 with type 1 diabetes mellitus(DM). Methods PCR amplification with sequence-specific primers was performed for HLA-DR2 genotyping in 64 type 1 DM patients(including 17 cases in group A with onset of diabetes before 15 years of age, 30 cases in group B between 15～30 and 17 cases in group C after 30) and 72 healthy controls. Results The frequency of HLA-DR2 in type 1 DM patients and controls was 31.3% and 36.6% respectively ( P > 0 05 ). The frequency of HLA-DR2 in group A, B and C was 17 6%, 30.0% and 47.1% respectively. The differences between any two subgroups of type 1 DM and between any subgroups of type 1 DM and control were not statistically significant ( P > 0.05 ). Comparing group A and group C resulted in P =0.058. The incidence of DK and/or DKA in the HLA-DR2 type 1 DM patients was lower than in the non-HLA-DR2 type 1 DM patients (55% and 84% respectively, P <0.025). Combined analysis showed a weak negative correlafion between HLA-DR2 and type 1 DM( P <0.025, RR=0.65, 95% CI 0.46～0.93).Conclusion To confirm the association between HLA-DR2 and type 1 DM in Chinese population, more studies with larger samples, especially in childhood onset type 1 DM patients are necessary. Furthermore, the association between HLA-DR2 and DK and/or DKA in type 1 DM patients remains to be verified.