GATA-3在鼠前房相关免疫偏离形成中的作用

GATA-3 in the development of anterior chamber associated immune deviation

目的 探讨GATA 3在前房相关免疫偏离 (anteriorchamberassociatedimmunedeviation ,ACAID)形成中的作用。方法 分别将视网膜光感受器间维生素A类结合蛋白 (interphotoreceptorretinoidbindingprotein ,IRBP)及其与完全弗氏佐剂混合液注入Wistar大鼠前房及鼠右后足垫内免疫动物 ,皮内注射IRBP观察迟发型超敏反应。分别于鼠前房内注射IRBP后的 3、5、7、14和 2 1d及鼠右后足垫内注射IRBP和完全弗氏佐剂混合液后 14d时处死动物 ,取其脾脏 ,采用免疫组织化学方法行单、双染色 ,对转录因子GATA 3的阳性表达细胞行免疫组织化学染色及Westernblot法检测 ,观察GATA 3在细胞中的表达水平。结果 正常大鼠脾脏组织细胞中 ,有少量GATA 3表达 ,前房注射IRBP的第 5、7、14及 2 1dGATA 3表达量显著增高 (P <0 0 1)。表达GATA 3的细胞主要是CD+ 4 T淋巴细胞。结论 GATA 3表达的增加早于ACAID的形成时间 (7d) ,提示GATA 3参与了ACAID形成 。

Objective To determine the role of GATA 3 (a transcript factor that has influences on Th2 cell differentiation) in the development of anterior chamber associated immune deviation (ACAID) Method Immunohistochemistry and Western blot were used to determin the localization and relative protein levels of GATA 3 respectively at different time points after anterior chamber injection of 50 μg interphotoreceptor retinoid binding protein (IRBP) Result GATA 3 was detected weakly in normal spleen and increased significantly at 5, 7, 14 and 21 days after anterior chamber inoculation It was detected mainly in CD + 4 T cells in spleen Conclusion The expression of GATA 3 increases earlier than development of ACAID It suggests that GATA 3 play an impormant role in Th2 commitment during ACAID development