4种肿瘤相关抗原在胆囊癌组织中的表达及其意义

Expression of 4 Kinds of Tumor-Associated Antigen in Gallbladder Carcinomas and Its Clinical Significance

目的 探讨肿瘤相关抗原表达对胆囊癌早期诊断及其预后判断的价值。方法 采用免疫组化染色对 10例慢性胆囊炎、10例胆囊腺瘤及 5 0例胆囊癌组织的癌胚抗原 (CEA)、癌相关粘液抗原 (CA5 0 )、钙粘附蛋白E(E CD)及增殖细胞核抗原 (PCNA )的表达进行检测。结果 胆囊癌组织中CEA及CA5 0表达阳性率显著高于腺瘤组和胆囊炎组(P<0 .0 5 ) ,而E CD表达阳性率明显降低 ,且伴有转移的胆囊癌E CD表达更低。胆囊癌的PCNA标记指数显著高于胆囊炎组和腺瘤组 (P<0 .0 1)。CEA及PCNA过度表达者 3年生存率显著降低 (P<0 .0 1) ,E CD过度表达者 3年生存率较阴性者明显增高 (P<0 .0 5 )。结论 CEA、CA5 0以及PCNA检测可能有助于胆囊腺瘤恶变和胆囊癌的早期诊断 ,且CEA、PCNA和E

Objective To explore the value of expression of carcinoma associated antigens in early diagnosis and predicting prognosis in gallbladder carcinoma. Methods The expression of carcinoembryonic antigen (CEA), carbohydrate antigen (CA50), E cadherin (E CD) and proliferating cell nuclear antigen (PCNA) in 10 cases of cholecystitis, 10 cases of gallbladder adenomas and 50 cases of gallbladder carcinomas were detected by immunohistochemistry. Results The positive rate of CEA, CA50 and PCNA labeling index (LI) in gallbladder carcinomas were significantly higher than that of gallbladder adenomas and cholecystitis ( P <0.05 and P <0.01). The positive rate of E CD in gallbladder carcinomas, especially with metastasis, was significantly lower than that of gallbladder adenomas and cholecystitis ( P < 0.05 ). The 3 year survival rate was significantly lower in gallbladder carcinomas with CEA and PCNA overexpression ( P < 0.05) , the 3 year survival rate in patients with E CD positive tumors was higher than that of those with negative tumors ( P < 0.05). Conclusion The detection of CEA, CA50 and PCNA is useful for early diagnosis of malignant change in gallbladder adenomas and gallbladder carcinomas. Therefore, the CEA, PCNA and E CD might be useful for predicting prognosis of gallbladder carcinomas.