摘要
目的 探讨分化诱导剂六亚甲基二乙酰胺 (hexamethylenebisacetamide ,HMBA)对人粘液表皮样癌细胞系MEC 1细胞增殖和凋亡相关基因表达的影响。方法 用 0 0 0 2mol/LHMBA对培养的MEC 1细胞进行体外诱导 72h后 ,分别采用光镜、免疫组化、原位杂交、图像分析等方法进行增殖细胞核抗原 (proliferatingcellnuclear,PCNA)、bax、bcl 2蛋白及mRNA表达水平的检测。结果 经HMBA诱导的MEC 1细胞中PCNA的表达较对照组明显下降 (P <0 0 1) ,同时MEC 1细胞的凋亡相关基因bax、bcl 2蛋白及mRNA表达均明显上升 (P <0 0 1)。结论 HMBA对MEC 1细胞的增殖基因PCNA有明显的负调控作用 ,从而可抑制其增殖 ;并且它能够特异性上调bax和bcl 2基因的表达 ,发挥了促进凋亡的作用。
Objective To investigate the effects of hexamethylene bisacetamide (HMBA) on related genes expression of apoptosis and cell proliferating in human mucoepidermoid carcinoma cell line MEC 1. Methods MEC 1 was induced 72 hours by 0 002 mol/L HMBA in vitro. The morphologic observation and the expression of PCNA, bcl 2,bax protein and bcl 2 mRNA were determined by microscope, immunohistochemistry, in situ hybridization and image analysis. Results The expression of bax、bcl 2 protein and bcl 2 mRNA were higher than control group ( P < 0 01). The related genes of cell proliferating PCNA were decreased in control group. Conclusions HMBA could inhibit the MEC 1 cell proliferation by down regulation of PCNA expression, it could also induce apoptosis effectively that might be through the way of up regulation of bax and bcl 2 gene expression.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2002年第2期120-122,T005,共4页
Chinese Journal of Stomatology
基金
甘肃省自然科学基金资助项目 (ZS991 A2 3 0 6 2 Y)
