脑星形细胞瘤组织中p16、bcl-2、bax蛋白的表达及其意义

Expression and Significance of p16 and bcl-2、bax Proteins in Astrocytomas

目的 探讨p16、bcl 2、bax蛋白在脑星形细胞瘤中的表达及其意义。方法 采用S P免疫组织化学法 ,检测p16、bcl 2、bax蛋白在 60例不同病理分级脑星形细胞瘤组织中的表达情况。结果 60例脑星形细胞瘤组织中 ,p16、bcl 2、bax蛋白表达的阳性率分别为 5 0 .0 % ( 30 /60 )、5 8.3% ( 35 /60 )、66.7% ( 40 /60 ) ,其中 p16蛋白阳性表达与病理分级有显著性相关 (P <0 .0 5 ) ;bcl 2、bax蛋白表达与病理分级无相关性 (P >0 .0 5 )。 60例脑星形细胞瘤组织中 ,p16、bcl 2、bax蛋白共同表达率 :Ⅰ级为 47.8% ( 11/2 3) ,Ⅲ～Ⅳ级为 0 ( 0 /16) ;在bcl 2、bax蛋白表达的基础上 ,不同病理分级组织中 p16蛋白的缺失率 :Ⅰ级为 4.3% ( 1/2 3) ,Ⅲ～Ⅳ级为 5 0 .0 % ( 8/16) ,有非常显著性差异 (P <0 .0 1)。结论 p16蛋白表达与脑星形细胞瘤的发生发展密切相关 ;在脑星形细胞瘤的发展过程中 ,bcl 2、bax、p16蛋白可能有某种协调作用。

Objective To study the expression of p16?bcl 2 and bax proteins,as well as their significance in the development and malignant progression of astrocytomas.Methods The expression of p16 protein and bcl 2?bax proteins were studied by S P immunohistochemistry in 60 cases of astrocytomas.Results p16 protein depletion was in accordance with the increasing grade of astrocytoma malignancy( P <0.05).The expression of bcl 2 and bax showed no significant correlation with grades of astrocytomas( P > 0.05 ).The co expression rate of p16,bcl 2 and bax was 47.8%(11/23) in astrocytomas grade I,and 0(0/16) in grade Ⅲ and Ⅳ. On the basis of the expression of bcl 2 and bax, the deletion rate of p16 was 4.3%(1/23) in astrocytomas grade I,and 50.0%(8/16) in grade Ⅲ and Ⅳ.( P <0.01).Conclusion The expression of p16 protein is associated with progression of astrocytomas.p16 protein and bcl 2,bax protein play a role in the oncogenesis and progression of astrocytomas.