胚胎大鼠神经干细胞体外培养分化的研究

In Vitro Expansion and Identification of Multipotent Neural Stem Cells from Embryonic Rats

为建立啮齿类动物中枢神经系统多潜能干细胞体外分离培养及分化鉴定的方法 ,将孕 1 8d胚胎大鼠海马及脑室下区组织分离 ,体外经EGF和bFGF刺激下扩增 ,然后撤除GFs培养 ;通过免疫细胞化学方法染色鉴定神经干细胞及其子代细胞的分化方向。结果 :培养的部分细胞在特定生长因子的作用下可分裂、复制 ,同时表达神经干细胞特异性抗原nestin ,并在撤除生长因子后向神经细胞和胶质细胞分化。结果表明 ,啮齿类动物海马及室下区存在着具有多向分化潜能的神经干细胞 ,其在神经发生或损伤过程中的作用尚需进一步探讨。

To establish the protocol of expansion and identification of multipotent stem cells from embryonic rodents central nervous system. Isolated cells from hippocampus and subventricular zone of E18 d rat were cultured in the presence of EGF and bFGF, which showed the self-renewing capability and differentiated to neurons and glial cells after withdrawing GFs. Immunocytochemical staining was used to identify stem cells and their progeny. Under appropriate conditions the neural stem cells not only undergo self-expansion and proliferation but also differentiate into cells, which exhibit characteristics of neuronal and astrocytic phenotypes. The present study showed the presence of multipotent stem cells in rodent hippocampus and subventricular zone of embryonic rats, but the role of them in neurogenesis or regeneration after injury remained to be investigated.