脑溢安干预大鼠脑出血对丝氨酸/苏氨酸激酶表达影响的研究

Effects of Nao YI An on Expression of Serine/Threonine Kinase Following Intracerebral Hemorrhage of Rats

目的 观察脑出血后丝氨酸／苏氨酸（Ｓｅｒｉｎｅ／ｔｈｒｅｏｎｉｎｅ Ｋｉｎａｓｅ Ａｋｔ）的表达及中药制剂脑溢安的干预作用。方法 用Ⅶ型胶原酶于脑内定位注射诱导大鼠脑出血模型，用免疫组织化学法观测脑内有Ａｋｔ表达的阳性神经元细胞与数目。结果 大鼠脑出血后１２ｈ时Ａｋｔ开始表达增加，４ｄ时Ａｋｔ达到高峰，７ｄ时仍有表达均高于假手术组（Ｐ＜０．０１）。脑溢安治疗组在脑出血后２４ｈ、２ｄ、４ｄ、７ｄ各时间点Ａｋｔ的表达均较模型组明显增高（Ｐ＜０．０５）。结论 大鼠脑出血后Ａｋｔ表达增加，脑溢安对Ａｋｔ有上调作用。

Objective To investigate the expression of Ak t aft er intracerebral hemorrhage(ICH) injury in rats and their expressions regula ted by Nao Yi An(NYA).Methods type Ⅶ collagenase was stereotaxi cally injecte d into the globas pallidus of rats,immunohistochemical analysis was employed to de tect the Akt in the brain tissues of rats.Results The expressions o f Akt in the model and NYA groups were increased after 12 h,peaked on d 4,and higher at 24h,d 2,d 4 and d 7 than those in the sham group.The Akt protein in NYA group was significantly higher than that in the model group at 24h?,d 2, d 4 and d 7 time points. Conclusions The level of Akt protein is in creased after ICH injury in the rat.NYA can markedly increase Akt expression.