99W·cm^(-2)脉冲微波辐照对大鼠血清激素水平影响的初步研究

A PRELIMINARY STUDY ON THE EFFECT OF 99W·CM^( -2) HIGH POWER PULSE MICROWAVE ON SERUM HORMONE LEVEL IN RATS

目的 :观察高功率脉冲微波 (HPPMW )辐照后大鼠血清中垂体、甲状腺、肾上腺及性腺部分激素的动态变化 ,以探讨HPPMW对内分泌系统的影响。方法 :二级雄性Wistar大鼠 35只 ,随机分为 7组 ,每组 5只 ,其中 1组为空白对照 ,其余 6组为辐照组。以功率为 99W·cm 2 脉冲微波辐照动物。于辐照后 1h、6h、2 4h、7d、14d及 2 8d分别采血 ,用放射免疫法在FT 6 30放免测定仪上测定血清睾丸酮 (testo)、雌二醇 (E2 )、促甲状腺激素 (TSH)、游离甲状腺素T4 (FT 4 )、皮质醇 (C0rt)、醛固酮 (Ald)及生长激素 (HGH)。所有数据经Spss 8.0统计处理。结果 :大鼠被辐照后 ,其血清E2在 1h～ 14d逐渐升高 ,在 2 8d则明显升高 (P <0 .0 1) ;Testo辐照后 1h、6h和 2 4h明显下降 (P <0 .0 5 ) ,2 8d则明显升高 (P <0 .0 1) ;TSH于辐照后逐渐升高 ,2 4h、14d到 2 8d时明显增高 (P <0 .0 5 ) ;FT 4辐照后 1h明显升高后 (P <0 .0 5 )逐渐降低 ,在 2 8d下降到最低点 (P<0 .0 1) ;Cort辐照后 1h ,2 4h和 14d升高 ,7d则明显降低 ;Ald于照后 2 4h明显升高 ,其它时相点均略低于对照组 ;HGH辐照后 1h～ 7d低于照前水平 ,其中 1h和 2 4h明显降低 (P <0 .0 5 ) ,但 2 8d又明显升高 (P <0 .0 1)。结论 :99W·cm 2

Objective:To observe the dynamic changes of some pituitary,adrenal, thyroid and sex hormones in serum of rats irradiated by 99 W·cm 2 high power pulse microwave (HPPMW),so as to explore its influence on endocrine system.Methods: 35 male Wistar rats were randomly divided into seven groups.Normal control group and six HPPMW groups. HPPMW groups were irradiated by 99 W·cm 2 HPPMW, then the left ventricle blood was obtained at 1 h,6 h,24 h,7 d,14 d and 28 respectively following irradiation, and serum testosterone (Testo),estradiol(E2),thyroid-stimulating hormone (TSH),free thyronin(FT4),cortisol (Cort),aldosterone (Ald) and human growth hormone (HGH) were measured by FT630 automatic radio-immunity analyzer. All data was analyzed by statistical software Spss 8.0.Results: serum E2 was incerased gradually at 1 h～14 d and increased significantly on 28 d(P<0.01); Testo was decreased significantly at 1 h, 6 h and 24 h (P<0.05); TSH was significantly increased at 24 h, 14 d and 28 d (P<0.05);FT4 was decreased gradually after increasing significantly at 1 h(P<0.05)and reached the lowest point on 29 d(P<0.05);Cort was increased at 1 h, 24 h and 14 d but decreased significantly on 7 d(P<0.05);Ald was increased significantly at 24 h(P<0.05)but were lower than that of control(P>0.05)in other periods.HGH was decreased significantly at 1 h and 24 h but reached the highest point on 28 d(P<0.01).Conclusion:HPPMW may have early durative effects on the levels of serum hormones in rats, indicating that it could diredtly induce injury to the endocrine system of rats.