胎羊体外循环中雌孕激素的变化

The Changes of Estradiol and Progesterone in Ewes during Fetal Cardiopulmonary Bypass

目的观察胎羊体外循环中妊娠激素的变化。 方法 10只怀孕山羊随机分为对照组 (n =4 )和胎羊体外循环组 (CPB组 ,n=6 )。对照组仅暴露胎羊心脏 ,不建立胎羊体外循环 ;CPB组建立胎羊常温体外循环 ,转流 1h。监测母羊和胎羊的心率、血压及血气值 ,运用放免技术检测转流前和转流 1h母羊血雌二醇、孕酮含量。结果实验中两组母羊心率、血压及血气值稳定 ,转流 1hCPB组母羊血雌二醇含量增加 ,孕酮含量下降 ,与对照组比较有显著性变化 (P <0 .0 5 )。停止体外循环 ,CPB组胎羊出现低氧、高碳酸血症和酸中毒 ,血压下降 ,心率减慢 (P<0 .0 5 )。对照组胎羊心率、血压及血气值无明显变化。 结论胎羊体外循环影响妊娠激素水平 。

Objective To study the changes of estradiol and progesterone in ewes during fetal cardiopulmonary bypass. Methods Ten ewes were divided into control group (n=4) and fetal cardiopulmonary bypass group (CPB group, n=6). The control group underwent fetal operative procedures without fetal cardiopulmonary bypass. CPB group underwent 1-hour-long fetal cardiopulmonary bypass. The heart rate, blood pressure, and blood gas of the ewes and fetal lambs were monitored during experimentation. The concentrations of estradiol and progesterone in the ewes'plasma were measured by radioimmunoassay at pre-bypass 1h during bypass. Results Ewes'heart rate, blood pressure and blood gas values were normal during experimentation. Estradiol increased significantly and progesterone decreased remarkably in CPB group comparing with the control group (P<0.05).After bypass, the fetal lambs of CPB group appeared hypoxic, hypercarbic and acidocic, while the heart rate and blood pressure decreased. But there were no changes in the fetal lambs of the control group. Conclusion Fetal cardiopulmonary bypass affects the levels of estradiol and progesterone of ewes. These changes may be harm to the fetal lambs.