葛根素、丹参注射液、川芎嗪对MCAO大鼠血浆6-keto-PGF_(1α)、TXB_2、NO、ET的影响

Study of Effect of Puerarin, Tetramethyl Pyrazine and Injection of Salvia Multiorrhiza Bunge. on Plasma 6 keto PGF 1α 、TXB 2、NO、ET in Rats wih Middle Cerebral Artery Occlusion

目的：研究ｉｖ 葛根素、川芎嗪 、丹参 注射液对大脑中动 脉结扎（ Ｍ Ｃ Ａ Ｏ） 后大鼠血 浆６ｋｅｔｏ Ｐ Ｇ Ｆ１α、 Ｔ Ｘ Ｂ２ 、 Ｎ Ｏ、 Ｅ Ｔ 的影响。方法：大鼠结扎大脑中动脉造成实验性脑缺血，ｉｖ 上述药物 １４ 天，应用 放免法及 硝酸酶还原法测定上述指标。结果：ｉｖ 葛 根素（５０ ｍ ｇ／ ｋｇ） 、丹参注射液（１５ｇ／ｋｇ） 、川芎嗪（２０ ｍ ｇ／ｋｇ） 均可增加 Ｍ Ｃ Ａ Ｏ 后６ｋｅｔｏ Ｐ Ｇ Ｆ１α与 Ｔ Ｘ Ｂ２ 比例，降低血浆 Ｅ Ｔ 的含量，葛根素还可明显降低血浆 Ｎ Ｏ 的浓度。结论： 上述药物 调节脑血流量改善微循环作用，主要与其对６ｋｅｔｏ Ｐ Ｇ Ｆ１α、 Ｔ Ｘ Ｂ２ 、 Ｎ Ｏ、 Ｅ Ｔ 的调节有关 。

Objective:In this study, the plsma 6 keto PGF 1α 、TXB 2、NO and ET in rats with middle cerebral artery occlusion (MCAO) were measured after intravenous injection of puerarin, tetramethyl pyrazine and Injection of Salvia multiorrhiza Bunge.Method:After MCAO, the rats were treated for 14 days with intravenous injection of these drugs.Those plsma factors were measured with radioimmunoassay method and nitric acid reductase methods.Result:Intravenous injection of puerarin(50mg/kg),tetramethyl pyrazine and Injection of Salvia multiorrhiza Bunge.can raise the ratio of 6 keto PGF 1α and TXB 2 and decrease the plasma ET.Intravenous injection of puerarin can decrease the plasma NO.Conclusion:The mechanism of increase cerebral blood flow and microcirculatory blood flow of those drugs is close related with their effects on plasma 6 keto PGF 1α ,TXB 2,NO and ET.