灵芝多糖对小鼠巨噬细胞内三磷酸肌醇和二酰基甘油的作用研究

Effect of Ganoderma polysaccharides on inositol trisphosphate and diacylglycerol in murine peritoneal macrophages

为研究灵芝多糖（ＧＬＢ７） 对小鼠腹腔巨噬细胞（ ＭΦ） 三磷酸肌醇（ＩＰ３） 和二酰基甘油（ＤＡＧ） 的作用，确定其对ＭΦ信号转导途径，采用细胞培养、放射免疫分析及离子交换层析和薄层层析法测定了小鼠腹腔ＭΦ中ＩＰ３ 和ＤＡＧ 的变化。结果显示ＧＬＢ７ 能引起小鼠ＭΦ中ＩＰ３ 和ＤＡＧ 浓度升高。ＩＰ３ 的达峰时间为３０ｓ，百日咳毒素（ＰＴＸ） 对此现象有抑制作用；ＤＡＧ 的合成出现两个峰，第一个峰迅速短暂，峰值位于３０ｓ，第二个峰是持续近２ｍｉｎ 的迟发峰。表明ＩＰ３／Ｃａ２ ＋ 和ＤＡＧ／ＰＫＣ

Objective: Through investigation the effect of GLB 7 on inositol trisphosphate (IP 3) and diacylglycerol (DAG) in murine peritoneal macrophages to provide a scientific evidence for it's immnomodulatory mechanism.Method: Cell culture, radioimmunological asssy, anion-exchange columns and thin-layer chromatography were used.Result: GLB 7 increased the production of IP 3 and DAG in murine peritoneal macrophages.The peak time of IP 3 was 30s, pertussis toxin (PTX) could inhibit GLB 7 from increasing the production of IP 3. In the production of DAG, two peak had been seen, the first peak was rapid and transitory, and the second was sluggish.Conclusion: The both path of signal transduction of IP 3/Ca 2+ and DAG/PKC are participated in the immunomodulatory of GLB 7 to macrophages.