华蟾素对三种消化系肿瘤细胞杀伤机制研究

Cytotoxicity of cinobufotalin on three digestive tract tumor cells in vitro

目的：研究华蟾素对体外培养的人胃癌、结肠癌、肝癌细胞的杀伤作用。方法：ＭＴＴ 法检测细胞增殖抑制率；光镜观察细胞形态改变；琼脂糖凝胶电泳观察ＤＮＡ 断裂。结果：三种肿瘤细胞在华蟾素（２ ｍｇ／ ｍｌ）孵育４８ 小时后其生长抑制率为１６－６ ％ ～２３－３ ％ ，细胞形态由多边形变成圆形，染色质疏松，细胞核溶解，ＤＮＡ电泳显示连续膜状条带。结论：华蟾素直接损伤肿瘤细胞ＤＮＡ。

Aim: To study the cytotoxicity of cinobufotalin on gastric, colonic and hepatocellar carcinoma cells in vitro.Methods: Inhibition of proliferation was measured with a colorimetric MTT assay.Morphological changes were observed by microscope, DNA fragmentation was visnalized by agarose gel electrophoresis.Results: After 48h incubation with cinobufotalin (2mg/ml), three tumor cells growth inhibition rate was 16.6%～23.3%. Cell changed from polygon into round.Chromatin looseness and karylysis were observed. DNA electrophoresis showed continuous band.Conclution: Cinobufotalin directly damage tumor cells DNA, which result in necrosis.