冰片加强川芎嗪抗小鼠急性缺氧作用

Enhancement of Synthetic Borneol on Ligustrazine Resisting Aoute Hypoxia in Mice

目的 :通过冰片 (SB)对川芎嗪 (Lig)的抗小鼠急性缺氧作用影响的研究 ,对药引的科学性进行探讨。 方法 :在小鼠急性缺氧装置上测定的耐缺氧能力 ,并测定心、脑中SOD、MDA和NO的含量。结果 :Lig可使小鼠的耐缺氧时间由 8 2± 0 7min增加至 1 1 4± 1 3min(P <0 .0 1 ) ,SB使Lig的小鼠抗缺氧作用增至 1 3 5± 2 1min(与川芎嗪比P <0 .0 5 ) ;Lig使心、脑的SOD含量分别增加 2 5 3%和 2 0 4% (P <0 .0 1 ) ,MDA含量分别降低 2 3 7%和 2 7 1 % ;加用SB后 ,Lig使心、脑的SOD含量分别比未用SB前增加1 8 1 %和 1 3 8% ,而MDA含量分别比未用SB前减少 2 2 6 %和 31 2 %。Lig使心、脑的NO含量分别由 5 84± 0 5 8μmol/g降至3 76± 0 32 μmol/g和由 3 5 7± 0 93μmol/g降至 2 1 1± 0 2 2 μmol/g ;使用SB后 ,Lig使心、脑的NO含量降低更明显。 结论 :Lig可增强小鼠的抗急性缺氧能力 ,使心、脑组织中SOD含量明显增加 ,MDA和NO含量显著降低 ,SB作为药引可使上述作用明显增强。

Objective: The science of yaoyin was probed into through studying the effect of synthetic borneol (SB) on ligustrazine (Lig) resisting acute hypoxia in mice. Methods: On the device of mice acute hypoxia, ability of tolerancing hypoxia of mice was investigated, and superoxide dismutase (SOD) and malondialdehyde (MDA) and nitric oxide (NO) contents in hearts and brains were measured. Results: Lig could make the action of resisting acute hypoxia in mice increased from 8.2±0.7min to 11.4±1.3min (P<0.01), SB made the action of resisting mice acute hypoxia of Lig change into 13.5±2.1min (compared with Lig Group, P<0.05): Lig made SOD contents in hearts and brains increase 25.3% and 20.4% respectively (P<0.01), MDA contents decreased 23.7% (heart) and 27.1% (brain) (P<0.01); after adding to SB, Lig could increase SOD contents and decrease MDA contents: Lig reduced NO contents from 5.84±0.58μmol/g (hearts) and 3.57±0.93μmol/g (brains) to 3.76±0.32μmol/g (hearts) and 2.11±0.22μmol/g (brains): after combined with SB, Lig made NO contents in hearts and brains significantly decrease. Conclusion: Lig had the action of resisting mice acute hypoxia, made SOD contents in mice heart and brain tissues significantly increaseed, but MDA and NO contents strikingly cut down; SB could make these actions evidently strengthen. As a yaoyin, SB could reinforce the action of Lig.