慢性糖尿病大鼠脑缺血再灌注损伤时纤溶酶原激活物及其抑制剂mRNA表达的动态变化

Dynamic Expressions of mRNA of Plasminogen Activators and Plasminogen Activator Inhibitors in Chronic Diabetic Rats with Cerebral Ischemial/Reperfusion

目的 观察正常和糖尿病大鼠脑缺血再灌注损伤时t PA、u PA、PAI 1和神经丝氨酸蛋白酶抑制剂基因表达的差异 ,探讨糖尿病加重脑缺血损伤的可能机制。方法 糖尿病大鼠和正常大鼠各 3 8只 ,随机分为脑缺血1h再灌注 1、2、5、11、2 3h组 (均n =6)、假手术组和非手术组 (均n =4 )。用RT PCR方法检测t PA、u PA、PAI 1和NSPmRNA表达。结果 糖尿病和正常大鼠脑缺血后t PA、u PA、PAI 1和NSPmRNA表达均增高 ;但糖尿病大鼠再灌注 2、11、2 3h时 ,缺血脑组织的NSPmRNA表达明显低于正常大鼠。结论 正常和糖尿病大鼠在脑缺血再灌注后存在纤溶酶原激活作用的增强 。

Purpose: To investigate the expressions of mRNA of plasminogen activators(PA) and plasminogen activator inhibitors(PAI) in cerebral ischemia/reperfusion rats with and without diabetes mellitus. Methods: Thirty-eight diabetic, 38 control rats were randomly divided into 7 subgroups according to different reperfusion time point(after being ischemia for 1 h, rats were followed reperfusion for 1,2,5,11 and 23 h respectively. n = 6 per subgroup), sham operation and non-operation (n = 4 per subgroup). The kinetic expressions of t-PA mRNA, u-PA mRNA, PAI-1 mRNA and NSP mRNA were measured by reverse transcription polymerase chain reaction(RT-PCR). Results: After cerebral ischemia/reperfusion, the expressions of t-PA, u-PA, PAI-1 and NSP mRNA increased in both groups. Reperfusion 2,11,23 h led to more significant decrease of NSP mRNA in diabetic than that in control group. Conclusions: After cerebral ischemia/reperfusion, plasminogen active function was increased in both diabetic and non-diabetic rats, but the mRNA expression of NSP was different.