T辅助淋巴细胞的功能变化在急性肺损伤中的意义

Function of T helper cell in mice with acute lung injury.

目的 观察急性肺损伤小鼠T辅助淋巴细胞 (TH) 1/TH2的功能变化 ,探讨其在急性肺损损伤发生中的作用。方法 小鼠腹腔注射脂多糖复制急性肺损伤模型。ELISA测定脾细胞培养上清液中干扰素 (1FN) -γ、白细胞介素 (IL) 4浓度 ,RT PCR测定细胞因子mRNA表达。伊文思蓝荧光法测定肺血管蛋白渗出量。结果 与正常对照组比较 ,急性肺损伤组动物肺组织中伊文思蓝渗出量、肺湿重 /干重比均明显升高。在刀豆素A刺激的脾细胞上清液中 ,急性肺损伤组IFN γ浓度显著下降 (P<0 0 1) ,IL 4明显升高 (P <0 0 1)。RT PCR显示急性肺损伤组动物脾细胞IFN γmRNA表达明显下降。结论 急性肺损伤时 ,THl向TH2漂移 ,以抗炎症反应为主 ,调控抗炎反应是急性肺损伤治疗的关键。

Objective To study the balance of TH1/TH2 cytokine in mice with acute lung injury(ALI).Methods Mice were randomly divided into ALI(n=8) and saline control groups(n=8).Interferon(IFN) γ and interleukin(IL) 4 were quantified by ELISA,mRNA expression was determined by RT PCR.The amount of Evans blue was measured by fluorescence method.Results As compared with control group,IFN γ in supernatants of cultured splenocyte was decreased (P<0 01),whereas IL 4 was increased(P<0 01)in ALI group.IFN γ mRNA expression was decreased in ALI group.As compared with control group,the lung wet to dry weight ratio and amount of Evan blue in lung were markedly increased in ALI group.Conclusions TH cell may shift from TH1 to TH2 type in ALI.Anti inflammatory response may be more important than inflammatory reaction in ALI. [