结肠癌患者自体瘤细胞对不同来源淋巴细胞功能的影响

AUTOLOGOUS TUMOR CELLS EFFECT THE IMMUNOLOGIC FUNCTION OF TIL,LNL AND PBL FROM COLORECTAL CANCER PATIENTS

目的 :研究结肠癌患者自体瘤细胞对肿瘤浸润淋巴细胞、肿瘤引流淋巴结的淋巴细胞、外周血淋巴细胞的影响。方法 :比较结肠癌患者自体肿瘤细胞对体外培养TIL、PBL、LNL细胞增殖、诱生细胞因子 (IL - 2、TNFα、IFNγ)水平的影响。结果 :发现结肠癌患者的TIL和PBL、LNL相比 ,CD8+细胞增加 ,CD4+细胞数减少。PBL和LNL对PHA、rhIL - 2刺激产生的细胞增殖能力比TIL强。PBL能产生较高水平的TNFα 和IFNγ,而IL - 2产生水平较低 ;LNL产生高水平的TNFα 和IL - 2 ,但仅产生低水平的IFNγ。TIL产生的TNFα、IFNγ 水平较PBL、LNL低。实验结果表明结肠癌患者自体肿瘤细胞对不同来源淋巴细胞的细胞因子分泌有不同影响 ,其机制有待进一步研究。

Objective:To study the effect of autologous tumor cells from colorectal cancer patients on the immunologic function of TIL,LNL and PBL.Methods:We had the diferent levels of autologic tumor cells on each lymphocyte in compasison.Results:TIL showed an enrichment of CD 8 + cells with a corresponding decrease in CD 4 + cells in comparison with PBL abd LNL.TIL produced lower levels of PHA and IL-2 upon mitogen/phorbol ester stimulation,while PBL produced high levels of TNF α and IFNr but low levels of IL-2. Under the same experimental conditions,LNL produced high levels of TNF α and IL-2 but low levels of IFN γ.TIL produced lower levels of TNF α and IFN γ than PBL and LNL did.Conclusion:Our results indicate different patterns of activities of TIL,LNL and PBL from colorectal-cancer patients.