脑溢安颗粒对脑出血大鼠脑组织bcl-2表达的影响

Effects of nao-yi-an granule on bcl-2 expression in the rat brain after experimental cerebral hemorrhage

目的 :探讨脑溢安颗粒 (简称脑溢安 )治疗脑出血的疗效机制。方法 :立体定位于苍白球内注射Ⅶ型胶原酶诱导大鼠脑出血模型 ,采用免疫组织化学法检测脑溢安组与各对照组脑组织内bcl 2蛋白表达。结果 :模型组脑组织内bcl 2阳性细胞在 1d开始增加 ,2d达峰值 ,7d时明显减少 ;脑溢安组其bcl 2阳性细胞在 2d ,4d ,7d明显高于模型组。结论 :脑溢安可上调bcl

Objective To investigate the protective effects and mechanisms of the traditional Chinese medicine complex, nao yi an granule (NYA) on experimental intracerebral hemorrhage (ICH) in rats. Methods ICH models anesthesized were induced by stereotaxical injection of 2μl normal saline containing 0.4U bacterial collagenase(type Ⅶ) into the right globas pallidus(GP), and the protein expression of bcl 2 in the rat brain after ICH was detected with the immunohistochemistry method, with which the effects of NYA were observed. Results Bcl 2 positive cells were found in the cerebral cortex and basal ganglia area after ICH. Immunoreactivity was localized in the cytoplasm as a fine granular precipitate. The levels of bcl 2 expression in the model group varied at different time points, and reached the peak at 2d. In the NYA group, the protein expression of bcl 2 was increased significantly both in the cortex and in the basal ganglia compared with those in the model group. Conclusion NYA can significantly upregulate the protein expression of bcl 2.