大肠良、恶性肿瘤Ki-67抗原的表达及其意义

Ki-67 antigen's expression in benign and malignant neoplasms of colorectum and its clinical significance

目的 :研究细胞增殖相关的核抗原 (Ki 6 7抗原 )在正常大肠粘膜以及大肠良、恶性肿瘤中的表达 ,探讨其临床意义。方法 :收集 33例大肠癌、13例大肠良性肿瘤和 13例正常粘膜的手术标本 ,用流式细胞术检测Ki 6 7抗原表达。结果 :大肠正常粘膜以及良、恶性肿瘤的Ki 6 7抗原标记指数 (Ki 6 7抗原阳性细胞比例 )分别为 2 .6 5 %± 1.5 1%、6 .38%± 2 .5 1%、18.5 2 %± 8.5 3%,组间两两比较均存在显著性差异 (P <0 .0 5 ) ;流式细胞仪检测Ki 6 7抗原表达鉴别大肠良、恶性肿瘤的灵敏度、特异性分别为 75 .76 %和 6 9.2 3%,Ki 6 7抗原表达在大肠癌的病理类型、Dukes分期、肿瘤体积、部位、病程、年龄、性别的组间两两比较 ,差异均没有显著性 (P >0 .0 5 )。结论 :流式细胞仪检测Ki 6 7抗原表达是大肠正常粘膜以及良、恶性肿瘤间相鉴别的一项有价值的指标。

Purpose:To study the expression of Ki-67 antigen in normal mucosae,benign and malignant neoplasms of colorectum and its clinical significance. Methods:33 cases of carcinomas,13 cases of benign neoplasms and 13 cases of normal mucosae of colorectum were collected. Ki-67 antigen was detected using flow cytometry.Results:The Ki-67 antigen positive fraction of normal mucosae,benign and malignant neoplasms of colorectum were 2.65%±1.51%,6.38%±2.51%,18.52%±8.53% respectivly, there being significant difference between the groups(P<0.05). In the differential diagnosis of benign and malignant neoplasms of colorectum, the sensitivity and specificity of Ki-67 antigen expression were 75.76%,69.23% respectivly, there was no statistical significance between Ki-67 antigen expression and clinical factors(P>0.05). Conclusions:Ki-67 antigen expression is a valuable marker to distinguish the normal mucosae,benign and malignant neoplasms of colorectum. Ki-67 antigen expression has no relation with tumor size,type of histopathology,Dukes stage,location,length of illness history,age,sex of the colorectal carcinoma patients.