亲细胞非均质分子脂质对人脑胶质瘤细胞系的抑制作用

Influence of cytotropic heterogenous molecular lipids(CHML) in human gliocytoma cell line

目的 :研究不同浓度亲细胞非均质分子脂质 (CHML)对人脑胶质瘤细胞的抑制作用。方法 :将指数生长期的SHG 4 4胶质瘤细胞分 6组 ,在 1～ 5组分别加入浓度为 5 0 0、2 0 0、10 0、5 0、2 0 μg/ml的CHML 6 .0和CHML 6 1复合液 ,第 6组加培养液作对照。于 2 4、48、72小时进行MTT检测 ,测定A值。结果 :各药物组与对照组的A值之间差别均有极显著的意义 ,其中两两比较检验表明 5 0 0 μg/ml、2 0 0 μg/ml组的A值与其他组比较差异有显著性。结论 :CHML对人脑胶质瘤细胞有显著抑制作用 ,并有一定剂量 效应关系 ,尤其是 5 0 0 μg/ml、2 0 0 μg/ml的药物组作用更为突出。

Purpose:To study the influence of cytotropic heterogenous molecular lipids (CHML) at different concentrations in human gliocytoma. Methods:The A values of SHG-44 gliocytoma which were divided into 6 groups were measured by MTT test at 24,48,72 hours. CHML of different concentrations were methylatted in the 1st～5th groups and inoculum was methylatted in the 6th group.Results:The A values of the 1st～5th groups were low compared with the 6th group. A significent difference was found in the groups in which the CHML concentration were 500 μg/ml and 200 μg/ml.Conclusions:These data indicate that CHML influenced gliocytoma significantly and there is correlation between dose and effect.The effect of CHML concentration of 500 μg/ml and of 200 μg/ml are prominent especially.