离子对高效液相色谱法测定复方甲硝唑片中甲硝唑和维生素B_6含量

Detertmination of metronidazole and vitamin B_6 in compound metronidazole tablets using ion-pair HPLC

目的 :建立同时测定复方甲硝唑片中甲硝唑和维生素B6 含量的离子对HPLC法。方法 :采用ODS色谱柱 ,磷酸二氢钾、庚烷磺酸钠溶液 (取磷酸二氢钾 1 3 .6g与庚烷磺酸钠1 .0 g ,加水溶解并稀释成 1 0 0 0ml) 甲醇 三乙胺 (85∶1 5∶0 .4) ,用磷酸调 pH至2 .8为流动相 ;检测波长 2 90nm ,峰面积外标法。结果 :甲硝唑和维生素B6 线性范围分别为 1 0 0～ 30 0 μg·ml- 1 (r =0 .9999) ,5～ 50 μ·ml- 1 (r=0 .9999) ;平均回收率分别为 1 0 0 .2 % (RSD =0 .93 % ) ,1 0 0 .4% (RSD =0 .44% ) ,n =6。结论 :离子对HPLC法测定复方甲硝唑片中甲硝唑和维生素B6 含量 ,方法准确 ,操作简便 。

OBJECTIVE To establish an ion pair HPLC method for determination of metronidazole and vitamin B 6 in compound metronidazole tablets.METHODS Column was used. The mobile phase was 0.1 mol·L -1 KH 2PO 4 methanol triethylamine (85∶15∶ 0.4 , adjust pH to 2.8 with phosphate acid), and the detective wavelength was 290 nm. RESULTS The linear ranges of metronidazole and vitamin B6 were 100～300 μg·ml -1 ( r = 0.9999 ) and 5～50 μg·ml -1 ( r = 0.9999 ), respectively; The average recovery were 100.2% (RSD= 0.93% ) and 100.4% (RSD= 0.44% ), n =6.CONCLUSIONS The method is accurate and simple with high recovery.