母牛分枝杆菌菌苗对糖尿病结核大鼠NO的影响

Effect of Mycobacterium Vaccae Vaccine on the Level of Nitric Oxide (NO) in Diabetic Rats Complicated with Tuberculosis

目的 :探讨母牛分枝杆菌菌苗对糖尿病并发结核大鼠的免疫调节机制。方法 :将Wistar大鼠随机分为 3组 :糖尿病结核 +母牛分枝杆菌菌苗免疫组 (A组 ) ,糖尿病结核组 (B组 ) ,结核组 (C组 )。链脲佐菌素腹腔注射建立糖尿病模型 ,A组皮内注射母牛分枝杆菌菌苗 5 0 μg ,1个月后所有大鼠尾静脉注射标准人型结核菌株H3 7Rv ,每 0 .4mL含0 .0 1mg。H3 7Rv感染 6周后抽血离心取上清液 ,测定一氧化氮 (NO)含量。结果 :与正常大鼠相比 ,糖尿病大鼠感染结核后NO水平含量明显降低 ;A组NO含量较B组明显升高。结论 :母牛分枝杆菌菌苗能提高糖尿病结核大鼠体内的NO水平。

Objective:To probe into the immunomodulatory mechanism of mycobacterium vaccae vaccine in diabetic rats complicated with tuberculosis. Methods:Wistar rats were randomly divided into three groups:Group A, diabetic rats complicated with tuberculosis+inoculation of Mycobacterium vaccae vaccine. Group B, diabetis rats complicated with tuberculosis; Group C, rats with tuberculosis; The animal model of diabetes was produced by intraperitoneal injection of STZ each animal of group C was given an intradermal injection of 50 μg of the vaccine. One month later, 0.4 mL (containing 0.01 mg) of standard human tubercle bacilli ( H 37 Rv strain) was injected intravenously into each rodent of all three groups. Six weeks after the H 37 Rv infection, blood samples were collected and the serum NO contents determined. Results:The levels of serum NO in diabetic rats complicated with tuberculosis were much lower than those in rats with tuberculosis only. However, the serum NO levels of animals in group A were significantly higher than group B animals. Conclusion:Mycobacterium vaccae vaccine is capable of elevating the level of NO in diabetic rats complicated with tuberculosis