自体移植静脉增生内膜内皮素I受体基因表达

Expression of endothelin A receptor gene ETAcDNA in proliferated intima of autogenous vein grafts

目的 应用内皮素 (ET) I型受体基因 (ETA c DNA)原位分子杂交的方法观察自体静脉移植血管平滑肌细胞(VSMC)增殖与内膜增生 (IH)的量效关系 ,并评价其在定量检测 VSMC增殖中的作用 .方法 家兔股动静脉移植模型 ,探针制备 ,原位分子杂交 ,微机图像分析 .结果 血管移植术后 1 W即有较多 ETAc DNA基因表达 ,术后 4 wk达最高峰 ,1 6wk和 48wk保持相对稳定 ,表达密度分别为 (1 8.4±5.8) % ,(42 .3± 3.6) % ,(42 .7± 7.2 ) %和 (43.1± 3.6) % ,IH相对厚度变化趋势与表达密度趋势相仿 ,1 ,4,1 6和 48wk分别为 (1 7.6± 5.2 ) % ,(53.0± 9.3) % ,(54.7± 8.7) %和(57.2± 1 0 .2 ) % ,经等级相关分析显示两者呈正相关 ,表达密度愈高则相对厚度愈小 .结论 VSMC增殖愈多对 IH贡献越大 ,应用 ETAc DNA基因探针定量分析自体静脉移植后增生内膜 VSMC变化准确特异 ;内皮素对血管

AIM To investigate the quantitative relationshipbetween vascular smooth muscle cell (VSMC) proliferation and intimal hyperplasia (IH) by using mRNA in situ hybridization with ETAcDNA (endothelin A receptor gene) probe, and to evaluate its role in quantitatively measuring VSMC proliferation. METHODS Model of femoral veins to arteries in rabbits was used. The gene probe was prepared, mRNA in situ hybridization selected and computerized image analysis system was applied. RESULTS There were quite a ETA gene expression positive in 1 wk postsurgery, and overexpression in 4 wk postsurgery which was the highest expression peak. The expression in 16 wk and 48 wk remained relatively stable as in 4 wk. Expression density in 1, 4, 16 and 48 wk groups respectively was (18.4± 5.8)%, (42.3± 3.6)%, (42.7±7.2)% and (43.1± 3.6)%. The trend of IH relative thickening was the same as that of expression density. The two measurements presented positive interaction through Spearman Rank correlation statistics analysis which demonstrated that the higher expression density, the thicker IH relative thickness. CONCLUSION In situ molecular hybridization with ETAcDNA gene probe is an accurate and specific method in quantitatively analyzing VSMC in IH. VSMC proliferation is a main part in development of IH. Endothelin may play an important role in IH of autogenous vein graft.