增殖瘢痕成纤维细胞接触后增殖及生物合成特性对异常瘢痕形成的机理

The characters of proliferating activity and biosynthetic function of fibroblasts from abnormal scars after complete contact in vitro

目的 为明确不同异常瘢痕成纤维细胞在体外完全接触后其增殖活性及生物合成功能的特性。方法 以瘢痕疙瘩、增生性瘢痕和正常皮肤 (各 6例 )为材料 ,通过细胞培养、免疫组织化学及分子生物学等方法 ,对不同成纤维细胞在细胞接触及未接触时通过检测增殖细胞核内抗原、P1 6 、Ⅰ、Ⅲ型胶原蛋白及前胶原基因表达对成纤维细胞的增殖、抑制及生物合成进行了研究。结果瘢痕疙瘩成纤维细胞接触后表现为细胞交叉重叠及较高的增殖活性及旺盛的生物合成功能 ,提示其失去了接触性抑制及密度抑制。皮肤成纤维细胞接触后则增殖及生物合成功能明显下降。增生性瘢痕成纤维细胞接触后表现为旺盛的生物合成功能 ,但其增殖活性处于瘢痕疙瘩和正常皮肤成纤维细胞之间。结论 不同瘢痕成纤维细胞接触后增殖及生物合成的特性可能是形成不同瘢痕的机理之一。

Objective\ This study was to determine the proliferating and biosynthetic characters of fibroblasts from abnormal scars and normal skins after complete contact in vitro. Methods\ 6 samples of keloid,hypertrophic scar and normal skin were collected respectively.Proliferation,inhibition and biosyntheses of different fibroblasts were investigated by detecting proliferating cell nuclear antigen(PCNA),P 16 ,type Ⅰ and Ⅲ collagen proteins,and Proα\-1(Ⅰ) and Proα\-1(Ⅲ) procollagen gene expressions under the condition of fibroblast complete contact by means of cell culture,immunohistochemistry and molecular biological techniques,etc. Results\ Overlap and high levels of proliferating activity and biosyntheses of complete contact fibroblasts from keloids indicated that these fibroblasts lost the behavior of contact inhibition and density inhibition.However,proliferating activity and biosynthetic function of complete contact fibroblasts from normal skins reduced dramatically.Biosynthetic function of complete contact fibroblasts from hypertrophic scars was still very strong,but proliferating activity was lower than keloid and higher than normal skin. Conclusion\ Proliferating and biosynthetic characters of complete contact fibroblasts from abnormal scars and normal skins were different,which may play an important role in the mechanism of abnormal scar formation.\;