反义TGF-β_1抑制Ⅲ度烫伤愈合中瘢痕形成的实验研究

The experimental study on antisense TGF-β_1 in inhibiting scar formation in Ⅲ° burn wound

目的 探讨反义TGF β1 对Ⅲ度烫伤愈合中瘢痕生成的抑制作用。方法 SD大鼠Ⅲ度烫伤后 ,分 3组进行处理 :①反义TGF β1 脱氧寡核苷酸 ;②反义TGF β1 重组质粒 ;③空白对照。分别于烫伤后 1,3,5 ,14dRT PCR、免疫组化检测TGF β1 mRNA和蛋白质的表达。烫伤后 5 ,14 ,2 1,2 8,6 0d原位杂交检测Ⅰ型胶原mRNA的表达变化 ,病理检测炎性细胞浸润反应和胶原分布状况。结果反义作用组在烫伤后 14d内TGF β1 mRNA和蛋白质的表达均减少。原位杂交显示对照组在 14dⅠ型胶原mRNA开始表达 ,2 8d达到高峰 ,随后减少 ,反义作用组无此现象 ,一直保持低表达。病理染色显示反义作用组炎性细胞浸润少 ,炎性反应低 ,胶原合成减少。结论 反义TGF β1

Objective\ To investigate the effect of antisense TGF β\-1 in inhibiting scar formation during wound healing. Methods\ SD rats were divided into three groups after skin burn:group one was treated with antisense TGF β\-1 oligonucleotide;group two was treated with antisense TGF β\-1 recombinant plasmid and the control group.In different time.RT\|PCR and immunohistochemistry were used to verify the expression of TGB β\-1 mRNA and protein.Type Ⅰ Collagen mRNA expression was determined by hybridization in situ.Inflammatory reaction and collagen distribution were observed pathologically. Results\ In the groups received antisense ODN and recombinant plasmid,the expression of TGF β\-1 mRNA and protein reduced during 14 days after burn.In the control group,type Ⅰ collagen mRNA began to express at the second week and reached a peak at the fourth week,while the antisense groups kept low expression.The antisense group also showed mild inflammatory reaction and less synthesis of collagen. Conclusion\ Antisense TGF\|β\-1 could prevent the scar formation during wound healing.\;