雷公藤内酯对睾丸切除雄性大鼠骨丢失的保护作用实验研究

Experimental study on the protective effect of triptolide on bone loss in male rats with testicular excision

目的探索雷公藤内酯对睾丸切除雄性大鼠的骨丢失保护作用。方法 30只3月龄的雄性大鼠随机分为3组:对照组(Sham组)、睾丸切除组(CON组)和雷公藤内酯治疗组(LGT组),每组10大鼠。行双侧睾丸切除手术或假手术,LGT组术后给予雷公藤内酯治疗12周,并在实验结束时安乐死大鼠,获取血清和大鼠胫骨。这些治疗的效果通过生物力学测试、Micro-CT扫描和血清生化分析进行评估。结果与CON组大鼠相比,雷公藤内酯的全身给药显著降低骨代谢指标[1型前胶原氨基末端前肽(type 1 procollagen amino terminal propeptide,P1NP)和Ⅰ型胶原羧基端肽(type I collagen carboxy terminal peptide,CTX-1)],增加大鼠胫骨骨密度,改善骨小梁参数(骨体积分数、骨小梁厚度、骨小梁数目和骨小梁分离度),增强胫骨极限载荷、能量和刚度,且比较差异有统计学意义(P<0.05)。结论雷公藤内酯能有效保护双侧切除睾丸大鼠诱导的骨丢失。

Objective To explore the protective effect of triptolide on bone loss in male rats with testicular excision. Methods Thirty 12-month-old male rats were randomly divided into 3 groups : control group (Sham group),testis resection group (CON group) and triptolide treatment group (LGT group),with 10 rats in each group. After bilateral orchiectomy or sham surgery,LGT group was treated with triptolide for 12 weeks and euthanized at the end of the experiment to obtain serum and rat tibia. The effects of treatments were evaluated by biomechanical tests,Micro-CT scans and serum biochemical analysis. Results Compared with untreated CON group rats, the systemic administration of triptolide significantly reduced the levels of bone metabolic markers [ type 1 procollagen amino terminal propeptide (P1NP) and type I collagen carboxy terminal peptide (CTX-1) ],increased bone mineral density (BMD),improved trabecular bone parameters in rats [ bone volume fraction (BV/TV),trabecular thickness (Tb. Th), trabecular number (Tb. N),and trabecular separation Degree (Tb. Sp)],and increased limit load of tibia,energy and stiffness,all reached statistically significance (P<0. 05). Conclusion Triptolide could effectively protect bone loss induced by bilateral resection of testes in rats.