温肾固疏方对绝经后骨质疏松大鼠骨代谢以及脂代谢的影响

The effect of warming kidney and retention formula on bone and lipid metabolism in rats with postmenopausal osteoporosis

目的探讨温肾固疏方改善骨质疏松和脂代谢异常的的作用机制。方法将60只大鼠的卵巢全部切除以建立绝经后骨质疏松模型,随机分为对照组(n=20)、雌激素组(n=20)和实验组(n=20)。假去势组(n=20)大鼠仅从卵巢周围切除少量脂肪。干预治疗8周后,采用酶联免疫法测定各组大鼠血清PING、TRACP5b、OPG、BGP的水平;并检测各组大鼠血脂指标。结果与假去势组大鼠比较,对照组大鼠的股骨和股骨近端的BMD明显降低(P<0.05);骨代谢指标和脂代谢指标水平均明显恶化(均P<0.05)。灌胃治疗后,与对照组比较,雌激素组和实验组大鼠的股骨和股骨近端的骨密度水平明显升高(P<0.05),并且两组大鼠的PING、TRACP5b、BGP水平均较显著提高(均P<0.05),而OPG水平显著降低(P<0.05)。此外,雌激素组和实验组大鼠的脂代谢指标水平均较对照组明显改善(均P<0.05)。结论温肾固疏方通过有效提高绝经后骨质疏松大鼠的骨密度,调节骨代谢和脂代谢指标水平,达到改善骨质疏松和调节血脂代谢的效果。

Objective To explore the mechanism of warming kidney and retention formula in relieving osteoporosis and lipid metabolic abnormality. Methods The ovaries of 60 rats were completely removed to establish postmenopausal osteoporosis model. The rats were then randomly divided into control group (n = 20), estrogen group (n = 20), and the experimental group (n = 20). A small amount of fat was removed from around the ovary only in rats of the false castration group (n=20). After 8 weeks of intervention, the serum levels of type I collagen amino end lengthening peptide (PING), osteoprotegerin (OPG), tartrate acid phosphatase-5b (TRACP5b), and osteocalcin (BGP) were measured using ELISA method. The blood lipid index was measured with an automatic analyzer. Results Compared with the rats in the false castration group, the BMD value of the femur and the proximal femur in the control group decreased significantly (P<0.05), and all the levels of bone metabolism index and lipid metabolism index deteriorated significantly (P<0.05). After gavage treatment, compared with the control group, the BMD value of the femur and the proximal femur in the estragen group and the experimental group increased significantly (P<0.05), and the levels of PING, TRACP5b, and BGP in both groups increased significantly (P<0.05), while the level of OPG reduced significantly (P<0.05). In addition, the levels of lipid metabolism in the estrogen group and the experimental group significantly improved than those in the control group (P<0.05). Conclusion Warming kidney and retention formula effectively improves bone mineral density, regulate bone metabolism and lipid metabolism, and achieve the effect of relieving osteoporosis and regulating blood lipid metabolism by effectively improving bone mineral density of postmenopausal osteoporotic rats.