活化素A在原发性干燥综合征患者中的表达分析研究

Expression analysis of activin A in patients with primary Sj?gren's syndrome

本研究旨在初步探讨活化素A在原发性干燥综合征(primary Sj?gren's syndrome, pSS)患者唇腺组织及外周血中的表达情况。选择上海交通大学医学院附属第九人民医院口腔黏膜科收治的60例pSS患者和30例对照者作为研究对象,采用ELISA检测血清活化素A的表达情况;采用实时荧光定量PCR分析PBMC中活化素A及相关受体mRNA的表达情况;采用免疫组织化学染色法分析唇腺组织样本中活化素A的表达情况。结果显示pSS患者血清及唇腺组织中活化素A的水平明显高于对照者(P <0.05);pSS患者PBMC活化素AβA亚基mRNA表达水平显著升高(P <0.01),但活化素Ⅰ型受体(activin receptorⅠ, ACVRⅠ)A和活化素Ⅱ型受体(activin receptorⅡ, ACVRⅡ)A的mRNA表达水平明显低于对照者(P <0.05),而ACVRⅠB、ACVRⅡB及其信号通路相关分子Smad2和Smad3的mRNA表达水平与对照者差异无统计学意义(P> 0.05)。研究结果表明活化素A在pSS患者组织和外周血中表达异常,可能参与了pSS的发生、发展。

We aimed to investigate the expression and role of activin A in patients with primary Sjogren's syndrome (pSS). 60 patients with pSS diagnosed in the Oral Mucosa Department of Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine and 30 controls were included. Serum levels of activin A were measured by ELISA. Expression of the activin A βA subunit, activin receptorⅠ(ACVRⅠ) A, ACVRⅡA, ACVRⅠB, ACVRⅡB and its signaling pathway-related molecules Smad2 and Smad3 in the PBMC of the patients and the healthy controls were analysed by real-time quantitative PCR. The expression of activin A in labial gland tissues was analysed by immunohistochemistry. The results indicated that activin A levels in the serum and labial gland tissues of pSS patients were significantly higher than those in the controls (P<0.05). Expression of activin A βA subunit in PBMC was significantly increased in the pSS patients(P <0.01), while ACVRⅠA and ACVRⅡA mRNA expressions in PBMC of the pSS patients were significantly decreased(P<0.05). There were no significant differences in the expression of ACVRⅠB, ACVRⅡB, Smad2 and Smad3 mRNA. Taken together, the abnormal expression of activin A in pSS patients implies that activin A might be involved in the pathogenesis of pSS.