Illumina MiSeq高通量测序降解黑龙江绥化地区玉米秸秆细菌菌群的研究

Bacterial Community on the Corn Straw in Suihua Area of Heilongjiang Province by Illumina Miseq Sequencing

以研究黑龙江绥化地区农产废弃物玉米秸秆细菌降解菌群为目的,采用Illumina MiSeq微生物多样性测序的方法,对2015～2017年玉米秸秆的菌群多样性进行测序。采用生物信息学的方法对低温自然降解秸秆细菌群落中主要降解菌进行分析,并筛选了2015～2017年玉米秸秆中两株丰度最高的纤维素降解细菌,经多次分离纯化并结合刚果红染色法验证其低温降解纤维素的功能。结果表明,测序获得61564385 bp,质控后共141219条16S rDNA序列,Silva数据库比对到14门、29纲、70目、136科、282属。其中假单胞菌属(Pseudomonas)、根瘤菌属(Rhizobium)、德沃西亚菌属(Devosia)、黄杆菌属(Flavobacterium)、微杆菌属(Microbacterium)和链霉菌属(Streptomyces)等是降解秸秆的主要细菌菌株,即该地区玉米秸秆细菌菌群多样性丰富,且研究筛选出的两株细菌具有纤维素降解能力。

The purpose of this paper was to study the bacterial degradation of corn straw in Suihua area of Heilongjiang province.Using the Illumina MiSeq microbial diversity sequencing method was used to sequence the microbial diversity of corn straw from 2015to 2017,and bioinformatics methods to main degrading bacteria in the bacterial community of straw biodegraded at low temperature were analyzed.And screened the two most abundant cellulose-degrading bacteria in corn straw from 2015to 2017.The functions of cellulose degradation at low temperature were verified by multiple separation and purification and combined with Congo red staining.The results showed that,the sequencing obtained 61564385bp ,and 141219 16S rDNA sequences after quality control.There were 14phylum,29class,70order,136family and 282genus with the Silva database.Among them,Pseudomonas,Rhizobium,Devosia,Flavobacterium,Microbacterium,Streptomyces and were the main bacterial strains for the degradation of straw.That was to say,the diversity of bacterial flora of corn straw in this area was abundant,and the two strains of bacteria screened by the study had the ability of cellulose degradation.