高良姜残渣黄酮的分离纯化及其抗氧化活性

Purification and Antioxidant Activity of Alpinia officinarum Residue Flavonoid

本研究以提取挥发油后的高良姜残渣为原料提取黄酮,采用7种大孔树脂进行静态吸附和解吸试验,筛选出最佳分离纯化树脂,再通过柱层析的动态吸附和洗脱试验,优化出分离纯化条件,并测定纯化前后的黄酮纯度和抗氧化活性。结果表明,XDA-6树脂最适合分离纯化高良姜黄酮,最佳纯化条件为上样流速2 BV/h,上样液浓度2 mg/m L,上样液体积31.6 BV,洗脱液为70%(v/v)乙醇,洗脱液流速2.5 m L/min,洗脱液用量3.1 BV,在此条件下,黄酮的纯度由43.55%±0.15%提高到85.42%±0.64%;纯化后的高良姜黄酮对DPPH与超氧阴离子自由基的清除率和还原能力均有所提升,清除DPPH和超氧阴离子的IC50值分别由纯化前的0.014、0.222 mg/m L降低到纯化后的0.012、0.186 mg/m L。

Capacities of static absorption and desorption of 7macroporous resins toward Alpinia officinarum flavonoids extracted from the Alpinia officinarum residue after essential oil extraction were investigated to select the fittest one with great result.Then dynamic absorption and desorption were carried out to find the best conditions of galangal flavonoids purification.Comparison of purity and antioxidant activity of the flavonoid before or after purification were used to estimate the effect of purification.Results showed that,XDA-6was the fittest macroporous resins for Alpinia officinarum flavonoid purification with the best absorption and desorption capacities,and the best condition of purification were as follows :Flow velocity of 2BV/h,sample concentration of 2mg/mL,sample volume of 31.6BV,the eluent was 70%(v/v)ethanol,the eluent flow rate was 2.5mL/min,and the eluent dosage was 3.1BV.Under this condition.The purity of the sample increased from 43.55%±0.15%to 85.42%±0.64%with the better scavenging rate of DPPH,scavenging rate of superoxide anion radicals and reducing ability.The IC50of DPPH and superoxide anion free radicals scavenging decreased from 0.014,0.222mg/mL to 0.012,0.186mg/mL.