低分子量瓜尔豆胶水解物对昆明小鼠便秘的预防作用

Preventive Effect of Low Molecular Weight Partially Hydrolyzed Guar Gum on Constipation in Kunming Mice

以复方地芬诺酯诱导的便秘昆明小鼠为模型,通过对小肠推进率、首粒黑便排出时间、6 h粪便湿重及个数、血清因子水平、小肠HE染色和粪便中有机酸含量等指标进行测定,研究低分子量瓜尔豆胶水解物(low molecular weight partially hydrolyzed guar gum简称L-PHGG)对便秘的预防效果。结果表明:相比于便秘模型组,L-PHGG低、中、高剂量组小鼠小肠推进率分别增加32.4%、54.4%和81.6%,阳性对照比沙可啶组增加57.0%; 6 h黑便湿重分别增加64.3%、79.1%和85.6%,比沙可啶组增加78.6%; P物质(SP)因子含量分别增加27.8%、44.1%和70.1%,比沙可啶组增加53.0%;血管活性肠肽(VIP)因子含量分别增加11.1%、9.3%和32.6%,比沙可啶组增加10.6%。各样品组小鼠小肠绒毛断裂较少,长度较长,数量更稠密,损伤程度较轻。乳酸、乙酸、丙酸三种有机酸总含量分别增加27.7%、35.3%和50.5%,比沙可啶组中仅有乳酸含量增加,增加量为25.3%。总之,L-PHGG低、中、高剂量均对昆明小鼠便秘具有预防作用,且中、高剂量组L-PHGG的便秘预防效果更好,为其开发成为胃肠道功能性食品提供科学依据。

This study was to evaluate the preventive effect of low molecular weight partially hydrolyzed guar gum( L-PHGG) on diphenoxylate-induced constipation in Kunming mice.The effects on the small intestinal propulsion rates,the defecation time of the first black stool,the wet weight and number of defecation within 6 hours after administration,serum parameters,HE staining of small intestine,and the contents of organic acids in feces were investigated. The results showed that compared with the constipation model mice,the mice those were fed L-PHGG at low,middle or high-dose presented in higher gastrointestinal transit,increased by 32.4%,54.4% and 81.6% respectively; and the positive control( bisacodyl group) increased by 57.0%.The wet weights of defecation within 6 hours after low,middle or high-dose of L-PHGG increased by 64.3%,79.1% and85.6% respectively,while the bisacodyl group increased by 78.6%.In addition,the levels of substance P( SP) of the L-PHGG at low,middle or high-dose increased by 27.8%,44.1% and 70.1% respectively,and the bisacodyl group increased by 53.0%.Serum vasoactive intestinal peptide( VIP) of the L-PHGG at low,middle or high-dose increased by 11.1%,9.3% and 32.6%respectively,and the bisacodyl group increased by 10.6%. HE staining of small intestinal showed that all L-PHGG groups presented less rupture,weaker damage,longer length and higher number of intestinal villi.The total content of lactic acid,acetic acid and propionic acid of low,middle or high-dose of L-PHGG increased by 27.7%,35.3% and 50.5% respectively,but the bisacodyl group only increased by 25.3%. In conclusion,these results suggested that L-PHGG of low,middle and high-dose had preventive effect on constipation in mice,especially for the middle and high-dose groups,which provides scientific supports for the development of L-PHGG as functional food for gastrointestinal tract.