山药化学成分研究

Chemical constituents from Dioscorea opposite

目的研究山药Dioscorea opposita化学成分。方法采用硅胶、Sephadex LH-20、反相ODS等柱色谱方法对化合物进行分离纯化,运用核磁共振波谱等方法鉴定化合物的结构。结果从山药根茎95%乙醇提取物中分离纯化得到14个化合物,包括8个二苯基庚烷类化合物(1～8)和6个含氮化合物(9～14),分别鉴定为5-ethoxy-1,7-diphenylheptan-3-one(1)、5-hydroxy-1,7-bis(4-hydroxyphenyl)-heptan-3-one(2)、1,7-diphenyl-4-hepten-3-one(3)、1,7-bis(4-hydroxyphenyl)-4-hepten-3-one(4)、hannokinol(5)、1-(4-hydroxy-3-methoxyphenyl)-7-(4-hydroxyphen-yl)-3,5-heptanediol(6)、1,7-bis(4-hydroxy-3-methoxyphenyl)-3,5-heptanediol(7)、1,7-bis(4-hydroxyphenyl)-1,5-epoxy-3-hydroxyheptane(8)、trans-N-coumaroyltyramine(9)、trans-N-feruloyltyramine(10)、cis-N-coumaroyltyramine(11)、trans-N-cinnamoyltyramine(12)、pyrrolezanthine-6-ethyl ether(13)、divaricataester A(14)。结论化合物1是新的天然产物,命名为山药庚酮A,化合物3、4、10、12～14为首次从薯蓣科植物中分离得到,化合物2、6、8为首次从山药中分离得到。

Objective To study the chemical constituents of Dioscorea opposita.Methods The constituents were isolated and purified by silica gel,Sephadex LH-20,and ODS column chromatography.Their structures were elucidated by NMR spectra comparison with reference data.Results Fourteen compounds were isolated from 95%ethanol extract of D.opposite.All of them included eight diarylheptanoids (1-8)and six nitrogen compounds (9-14).They were elucidated by spectroscopic data as 5-ethoxy-1,7-diphenylheptan-3-one (1),5-hydroxy-1,7-bis(4-hydroxyphenyl)-heptan-3-one (2),1,7-diphenyl-4-hepten-3-one (3), 1,7-bis (4-hydroxyphenyl)-4-hepten-3-one (4),hannokinol (5),1-(4-hydroxy-3-methoxyphenyl)-7-(4-hydroxyphenyl)-3,5-heptanediol (6), 1,7-bis (4-hydroxy-3-methoxyphenyl)-3,5-heptanediol (7),1,7-bis(4-hydroxyphenyl)-1,5-epoxy-3-hydroxyheptane (8),trans-N- coumaroyltyramine (9),trans-N-feruloyltyramine (10),cis-N-coumaroyltyramine (11),trans-N-cinnamoyltyramine (12), pyrrolezanthine-6-ethyl ether (13),divaricataester A (14).Conclusion Compounds 1is a new natural product,named as dioscoheptone A.Compounds 3,4,10,12,13,and 14are isolated from the family of dioscoreaceae for the first time.Compounds 2,6, and 8are isolated from D.opposita for the first time.